Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85

Koenig, Patrick, Mirus, Oliver, Haarmann, Raimund, Sommer, Maik S., Sinning, Irmgard, Schleiff, Enrico and Tews, Ivo (2010) Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85. Journal of Biological Chemistry, 285, (23), 18016-18024. (doi:10.1074/jbc.M110.112649). (PMID:8985185).


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Proteins of the Omp85 family are conserved in all kingdoms of life. They mediate protein transport across or protein insertion into membranes and reside in the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Omp85 proteins contain a C-terminal transmembrane beta-barrel and a soluble N terminus with a varying number of polypeptide-transport-associated or POTRA domains. Here we investigate Omp85 from the cyanobacterium Anabaena sp. PCC 7120. The crystallographic three-dimensional structure of the N-terminal region shows three POTRA domains, here named P1 to P3 from the N terminus. Molecular dynamics simulations revealed a hinge between P1 and P2 but in contrast show that P2 and P3 are fixed in orientation. The P2-P3 arrangement is identical as seen for the POTRA domains from proteobacterial FhaC, suggesting this orientation is a conserved feature. Furthermore, we define interfaces for protein-protein interaction in P1 and P2. P3 possesses an extended loop unique to cyanobacteria and plantae, which influences pore properties as shown by deletion. It now becomes clear how variations in structure of individual POTRA domains, as well as the different number of POTRA domains with both rigid and flexible connections make the N termini of Omp85 proteins versatile adaptors for a plentitude of functions.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1074/jbc.M110.112649
ISSNs: 0021-9258 (print)
1083-351X (electronic)
Keywords: bacteria, evolution, membrane proteins, protein structure, protein translocation, potra domains, membrane biogenesis
Subjects: Q Science > QR Microbiology
Divisions : Faculty of Natural and Environmental Sciences > Biological Sciences
ePrint ID: 200239
Accepted Date and Publication Date:
4 June 2010Published
26 March 2010Made publicly available
Date Deposited: 24 Oct 2011 12:23
Last Modified: 31 Mar 2016 13:45

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