A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens
Rice, Jason and Christodoulides, Myron (2011) A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens. Methods in Molecular Biology, 799, 405-419. (doi:10.1007/978-1-61779-346-2_22) (PMID:21993657)
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Description/Abstract
DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.
| Item Type: | Article |
|---|---|
| ISSN: | 1064-3745 (print) 1940-6029 (electronic) |
| Uncontrolled Keywords: | dna vaccine, neutralizing humoral antibody, t cell help, infectious disease |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine > Cancer Sciences |
| ePrint ID: | 200257 |
| URI: | http://eprints.soton.ac.uk/id/eprint/200257 |
| Deposited On: | 24 Oct 2011 15:33 |
| Last Modified: | 24 Oct 2011 15:33 |
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