A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens
Rice, Jason and Christodoulides, Myron (2011) A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens. Methods in Molecular Biology, 799, 405-419. (doi:10.1007/978-1-61779-346-2_22). (PMID:21993657).
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DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.
|Digital Object Identifier (DOI):||doi:10.1007/978-1-61779-346-2_22|
|Keywords:||dna vaccine, neutralizing humoral antibody, t cell help, infectious disease|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology
R Medicine > RB Pathology
|Divisions:||Faculty of Medicine > Cancer Sciences
|Date Deposited:||24 Oct 2011 14:33|
|Last Modified:||27 Mar 2014 19:46|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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