A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens

Rice, Jason and Christodoulides, Myron (2011) A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens. Methods in Molecular Biology, 799, 405-419. (doi:10.1007/978-1-61779-346-2_22). (PMID:21993657).


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DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1007/978-1-61779-346-2_22
ISSNs: 1064-3745 (print)
1940-6029 (electronic)
Keywords: dna vaccine, neutralizing humoral antibody, t cell help, infectious disease
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RB Pathology
Divisions : Faculty of Medicine > Cancer Sciences
ePrint ID: 200257
Accepted Date and Publication Date:
19 October 2011Published
Date Deposited: 24 Oct 2011 14:33
Last Modified: 31 Mar 2016 13:45
URI: http://eprints.soton.ac.uk/id/eprint/200257

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