Electrokinetic DNA transport in 20 nm-high nanoslits: Evidence for movement through a wall-adsorbed
Castillo-Fernandez, Oscar, Salieb-Beugelaar, Georgette B., Bomer, Johan G., Arundell, Martin, Samitier, Josep, van den Berg, Albert and Eijkel, Jan C.T. (2011) Electrokinetic DNA transport in 20 nm-high nanoslits: Evidence for movement through a wall-adsorbed. Electrophoresis, 32, (18), 2402-2409. (doi:10.1002/elps.201100278) (PMID:21922490)
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Description/Abstract
The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP (‘nano-gel’) can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
| Item Type: | Article |
|---|---|
| ISSN: | 0173-0835 (print) 1522-2683 (electronic) |
| Uncontrolled Keywords: | λ-dna, biased reptation, caterpillar movement, nanoslits, pathways, pvp |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Natural and Environmental Sciences > Biological Sciences |
| ePrint ID: | 200275 |
| URI: | http://eprints.soton.ac.uk/id/eprint/200275 |
| Deposited On: | 24 Oct 2011 17:01 |
| Last Modified: | 02 Mar 2012 12:18 |
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