Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed
Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed
The electrokinetic transport behavior of ?-DNA (48?kbp) in 20?nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP (‘nano-gel’) can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
?-dna, biased reptation, caterpillar movement, nanoslits, pathways, pvp
2402-2409
Castillo-Fernandez, Oscar
3b88aa60-ab90-4a85-bde2-4f380d75ccbf
Salieb-Beugelaar, Georgette B.
62a65a50-be5d-48fd-b273-7a1f78de16e7
Bomer, Johan G.
dd026567-bd3d-46ad-a6bd-893412aada88
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Samitier, Josep
7735286e-85a5-4b4d-b8af-2826e1441b77
van den Berg, Albert
3cd94d32-26da-41a1-8505-18b7cd3edcbd
Eijkel, Jan C.T.
52cfa014-54ac-42b1-ad97-d1221c560f9f
September 2011
Castillo-Fernandez, Oscar
3b88aa60-ab90-4a85-bde2-4f380d75ccbf
Salieb-Beugelaar, Georgette B.
62a65a50-be5d-48fd-b273-7a1f78de16e7
Bomer, Johan G.
dd026567-bd3d-46ad-a6bd-893412aada88
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Samitier, Josep
7735286e-85a5-4b4d-b8af-2826e1441b77
van den Berg, Albert
3cd94d32-26da-41a1-8505-18b7cd3edcbd
Eijkel, Jan C.T.
52cfa014-54ac-42b1-ad97-d1221c560f9f
Castillo-Fernandez, Oscar, Salieb-Beugelaar, Georgette B., Bomer, Johan G., Arundell, Martin, Samitier, Josep, van den Berg, Albert and Eijkel, Jan C.T.
(2011)
Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed.
Electrophoresis, 32 (18), .
(doi:10.1002/elps.201100278).
(PMID:21922490)
Abstract
The electrokinetic transport behavior of ?-DNA (48?kbp) in 20?nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP (‘nano-gel’) can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
This record has no associated files available for download.
More information
e-pub ahead of print date: 26 August 2011
Published date: September 2011
Keywords:
?-dna, biased reptation, caterpillar movement, nanoslits, pathways, pvp
Organisations:
Centre for Biological Sciences
Identifiers
Local EPrints ID: 200275
URI: http://eprints.soton.ac.uk/id/eprint/200275
ISSN: 0173-0835
PURE UUID: 69a95a66-7a54-413a-b097-8c370d9b2c5b
Catalogue record
Date deposited: 24 Oct 2011 16:01
Last modified: 14 Mar 2024 04:19
Export record
Altmetrics
Contributors
Author:
Oscar Castillo-Fernandez
Author:
Georgette B. Salieb-Beugelaar
Author:
Johan G. Bomer
Author:
Josep Samitier
Author:
Albert van den Berg
Author:
Jan C.T. Eijkel
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics