NMR redox studies of flavocytochrome c(3) from Shewanella frigidimarina
NMR redox studies of flavocytochrome c(3) from Shewanella frigidimarina
Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages.
shewanella, electron tranfer protein, multihaem, flavocytochrome, nmr, paramagnetic shiftsfumarate reductase, cytochrome c(3), desulfovibrio-vulgaris, energytransduction, putrefaciens mr-1, electron-transfer, heme core, cooperativity, respiration, ncimb400
379-381
Pessanha, M.
4314f0ea-71a6-49f0-b68d-0f3ae8a74fba
Turner, D. L.
158980fd-3487-480b-baba-15102dfb64b4
Rothery, E. L.
f6a59997-8e13-4c10-bcfc-340c08fc53e5
Pankhurst, K. L.
3d9eb18b-69ee-4088-a760-b007912b3723
Reid, G. A.
1120e44f-b6db-4b84-bcf8-c58a20df7585
Chapman, S. K.
69bd0a6b-3dc1-44e5-97e4-bca3808c52e9
Xavier, A. V.
b73ea5e9-4b82-441a-96e8-7907c5fab76b
Salgueiro, C. A.
f2558f8c-9e4d-4f20-8c36-9a77e86e23c7
3 December 2003
Pessanha, M.
4314f0ea-71a6-49f0-b68d-0f3ae8a74fba
Turner, D. L.
158980fd-3487-480b-baba-15102dfb64b4
Rothery, E. L.
f6a59997-8e13-4c10-bcfc-340c08fc53e5
Pankhurst, K. L.
3d9eb18b-69ee-4088-a760-b007912b3723
Reid, G. A.
1120e44f-b6db-4b84-bcf8-c58a20df7585
Chapman, S. K.
69bd0a6b-3dc1-44e5-97e4-bca3808c52e9
Xavier, A. V.
b73ea5e9-4b82-441a-96e8-7907c5fab76b
Salgueiro, C. A.
f2558f8c-9e4d-4f20-8c36-9a77e86e23c7
Pessanha, M., Turner, D. L., Rothery, E. L., Pankhurst, K. L., Reid, G. A., Chapman, S. K., Xavier, A. V. and Salgueiro, C. A.
(2003)
NMR redox studies of flavocytochrome c(3) from Shewanella frigidimarina.
Inorganica Chimica Acta, 356, .
(doi:10.1016/S0020-1693(03)00179-8).
Abstract
Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages.
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Published date: 3 December 2003
Keywords:
shewanella, electron tranfer protein, multihaem, flavocytochrome, nmr, paramagnetic shiftsfumarate reductase, cytochrome c(3), desulfovibrio-vulgaris, energytransduction, putrefaciens mr-1, electron-transfer, heme core, cooperativity, respiration, ncimb400
Identifiers
Local EPrints ID: 20064
URI: http://eprints.soton.ac.uk/id/eprint/20064
ISSN: 0020-1693
PURE UUID: 09fe34a1-d9c9-4a2d-ad80-f2b2ffb5cab5
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Date deposited: 23 Feb 2006
Last modified: 15 Mar 2024 06:21
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Contributors
Author:
M. Pessanha
Author:
D. L. Turner
Author:
E. L. Rothery
Author:
K. L. Pankhurst
Author:
G. A. Reid
Author:
S. K. Chapman
Author:
A. V. Xavier
Author:
C. A. Salgueiro
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