Probing the interaction of lipids with the non-annular binding sites of the potassium channel KcsA by magic-angle spinning NMR


Marius, Phedra, de Planque, Maurits R.R. and Williamson, Philip T.F. (2012) Probing the interaction of lipids with the non-annular binding sites of the potassium channel KcsA by magic-angle spinning NMR. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1818, (1), 90-96. (doi:10.1016/j.bbamem.2011.09.017). (PMID:21963409).

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Description/Abstract

The activity of the potassium channel, KcsA is tightly regulated through the interactions of anionic lipids with high-affinity non-annular lipid binding sites located at the interface between the channel's subunits. Here we present solid-state phosphorous NMR studies that resolve the negatively charged lipid phosphatidylglycerol within the non-annular lipid-binding site. Perturbations in chemical shift observed upon the binding of phosphatidylglycerol are indicative of the interaction of positively charged sidechains within the non-annular binding site and the negatively charged lipid headgroup. Site directed mutagenesis studies have attributed these charge interactions to R64 and R89. Functionally the removal of the positive charges from R64 and R89 appears to act synergistically to reduce the probability of channel opening

Item Type: Article
ISSNs: 0005-2736 (print)
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Natural and Environmental Sciences > Biological Sciences > Molecular & Cellular
Faculty of Natural and Environmental Sciences > Chemistry > Magnetic Resonance
ePrint ID: 201215
Date Deposited: 28 Oct 2011 10:50
Last Modified: 27 Mar 2014 19:47
URI: http://eprints.soton.ac.uk/id/eprint/201215

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