DNA sequence recognition by an isopropyl substituted thiazole polyamide

James, Peter L., Merkina, Elena E., Khalaf, Abedawn I., Suckling, Colin J., Waigh, Roger D., Brown, Tom and Fox, Keith R. (2004) DNA sequence recognition by an isopropyl substituted thiazole polyamide. Nucleic Acids Research, 32, (11), 3410-3417. (doi:10.1093/nar/gkh666).


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Original Publication URL: http://dx.doi.org/10.1093/nar/gkh666


We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1093/nar/gkh666
ISSNs: 0305-1048 (print)
Related URLs:
Keywords: minor-groove, hairpin polyamide, binding, drug, duplex, specificity, distamycin, netropsin, ligands
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 20246
Accepted Date and Publication Date:
1 June 2004Published
Date Deposited: 21 Feb 2006
Last Modified: 31 Mar 2016 11:37
URI: http://eprints.soton.ac.uk/id/eprint/20246

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