Thiamine biosynthesis in Escherichia coli: in vitro reconstitution of the thiazole synthase activity
Leonardi, Roberta and Roach, Peter L. (2004) Thiamine biosynthesis in Escherichia coli: in vitro reconstitution of the thiazole synthase activity. Journal of Biological Chemistry, 279, (17), 17054-17062. (doi:10.1074/jbc.M312714200).
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The biosynthesis of thiamine in Escherichia coli requires the formation of an intermediate thiazole from tyrosine, 1-deoxy-D-xylulose-5-phosphate (Dxp), and cysteine using at least six structural proteins, ThiFSGH, IscS, and ThiI. We describe for the first time the reconstitution of thiazole synthase activity using cell-free extracts and proteins derived from adenosine-treated E. coli 83-1 cells. The addition of adenosine or adenine to growing cultures of Aerobacter aerogenes, Salmonella typhimurium, and E. coli has been shown previously to relieve the repression by thiamine of its own biosynthesis and increase the expression levels of the thiamine biosynthetic enzymes. By exploiting this effect, we show that the in vitro thiazole synthase activity of cleared lysates or desalted proteins from E. coli 83-1 cells is dependent upon the addition of purified ThiGH-His complex, tyrosine (but not cysteine or 1-deoxy-D-xylulose-5- phosphate), and an as yet unidentified intermediate present in the protein fraction from these cells. The activity is strongly stimulated by the addition of S-adenosylmethionine and NADPH.
|Keywords:||enterica serovar typhimurium, fe-s cluster, salmonella-typhimurium, 5-aminoimidazole ribotide, pyrimidine moiety, nifs gene, protein, identification, precursor, complex|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||University Structure - Pre August 2011 > School of Chemistry
|Date Deposited:||20 Feb 2006|
|Last Modified:||01 Jun 2011 11:05|
|Contributors:||Leonardi, Roberta (Author)
Roach, Peter L. (Author)
|Date:||23 April 2004|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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