Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA


Osborne, Sadie D., Powers, Vicki E.C., Rusling, David A., Lack, Oliver, Fox, Keith R. and Brown, Tom (2004) Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA. Nucleic Acids Research, 32, (15), 4439-4447. (doi:10.1093/nar/gkh776).

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Original Publication URL: http://dx.doi.org/10.1093/nar/gkh776

Description/Abstract

We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-forming oligonucleotides retain exquisite sequence specificity, with enhanced discrimination against YR base pairs (especially CG). These properties make BAU a useful base analogue for the sequence-specific creation of stable triple helices at pH 7.0.

Item Type: Article
ISSNs: 0305-1048 (print)
Related URLs:
Keywords: double-stranded dna, c+center-dot-gc, helix formation, -aminoethoxy-modified oligonucleotides, dual recognition, gene-expression, stability, stabilization, spectroscopy, polyamines
Subjects: T Technology > TP Chemical technology
Q Science > QH Natural history > QH426 Genetics
Divisions: University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 20299
Date Deposited: 20 Feb 2006
Last Modified: 27 Mar 2014 18:10
URI: http://eprints.soton.ac.uk/id/eprint/20299

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