Chemical modification and micropatterning of Si(100) with oligonucleotides


Yin, H.B., Brown, T., Greef, R., Wilkinson, J.S. and Melvin, T. (2004) Chemical modification and micropatterning of Si(100) with oligonucleotides. Microelectronic Engineering, 73-73, 830-836. (doi:10.1016/j.mee.2004.03.060).

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Description/Abstract

A method for the reliable attachment. of oligonucleotides to silicon (10 0) surfaces in patterns is described. LTV light exposure of silicon (10 0) coated with alkenes functionalized with carboxylic acid groups and derivatives results in covalent attachment to the silicon surface. The carboxyl-terminated (and derivatives) surfaces act as a substrate for the chemistry of the subsequent attachment of oligonucleotides. Illustrated are our results gained for optimisation of the surface attachment chemistry and the characterisation of the surface with scanning electron microscopy, epifluorescence microscopy and ellipsometry.

Item Type: Article
ISSNs: 0167-9317 (print)
Related URLs:
Keywords: silicon functionalisation, oligonucleotide, conjugation, surface attachment chemistryself-assembled monolayers, alkyl monolayers, surfaces, silicon, 1-alkenes, proteins, cells, gold
Subjects: T Technology > TP Chemical technology
Q Science > QD Chemistry
Q Science > QC Physics
Divisions: University Structure - Pre August 2011 > Optoelectronics Research Centre
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 20345
Date Deposited: 16 Feb 2006
Last Modified: 27 Mar 2014 18:10
URI: http://eprints.soton.ac.uk/id/eprint/20345

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