Interleukin-13, but not indomethacin, increases cysteinyl-leukotriene synthesis in human lung macrophages
Jackson, Sarah E., Holloway, John W., Warner, Jane A. and Sampson, Anthony P. (2011) Interleukin-13, but not indomethacin, increases cysteinyl-leukotriene synthesis in human lung macrophages. Journal of Allergy, 1-6. (doi:10.1155/2012/348741). (PMID:22121385).
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Aspirin-exacerbated respiratory disease (AERD) is associated with constitutively elevated synthesis of bronchoconstrictor cysteinyl-
leukotrienes, associated with increased expression of leukotriene (LT)C4 synthase and Th2 cytokines and airway eosinophilia.
We examined whether interleukin-13 can increase LTC4 synthase gene transcription and cysteinyl-leukotriene synthesis in
macrophages isolated from resected human lung tissue and whether an NSAID (indomethacin) can trigger further cysteinylleukotriene
synthesis in these cells. Overnight culture of human lung macrophages with IL-13 (10 ng/mL) increased spontaneous
and ionophore-stimulated production of cysteinyl-leukotrienes by 42% (P = 0.02) and 52% (P = 0.005), respectively, as quantified
by enzyme immunoassays, but PCR gene transcription assays did not demonstrate an effect on LTC4S mRNA. The addition of
indomethacin (100 μM) did not modulate cysteinyl-leukotriene production in either IL-13-treated or untreated macrophages.We
conclude that while IL-13 enhances cysteinyl-leukotriene synthesis in human lung macrophages, it does not replicate the enhanced
LTC4 synthase expression observed in the AERD lung nor confer sensitivity to NSAIDs.
|Subjects:||Q Science > QH Natural history > QH426 Genetics
R Medicine > R Medicine (General)
|Divisions:||Faculty of Medicine > Human Development and Health
Faculty of Medicine > Infection, Inflammation and Immunity
|Date Deposited:||30 Nov 2011 14:43|
|Last Modified:||30 Nov 2012 17:43|
|Contributors:||Jackson, Sarah E. (Author)
Holloway, John W. (Author)
Warner, Jane A. (Author)
Sampson, Anthony P. (Author)
|Date:||29 October 2011|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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