2′-aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation
Lou, Chenguang, Shelbourne, Montserrat, Fox, Keith R. and Brown, Tom (2011) 2′-aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. Chemistry - A European Journal, 17, (52), 14851-14856. (doi:10.1002/chem.201102287).
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Description/Abstract
The phosphoramidite monomer of the C-nucleoside 2′-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistance on oligonucleotides. TFOs containing AE-MAP have potential for gene knock-out and gene expression studies.
| Item Type: | Article |
|---|---|
| ISSNs: | 0947-6539 (print) 1521-3765 (electronic) |
| Related URLs: | |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Natural and Environmental Sciences > Biological Sciences > Molecular & Cellular |
| Item ID: | 206549 |
| Date Deposited: | 22 Dec 2011 16:32 |
| Last Modified: | 22 Dec 2011 16:32 |
| Contributors: | Lou, Chenguang (Author) Shelbourne, Montserrat (Author) Fox, Keith R. (Author) Brown, Tom (Author) |
| Date: | 2011 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/206549 |
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