Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat
Brown, Nicholas M., Rachwal, Phillip A., Brown, Tom and Fox, Keith R. (2005) Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat. Organic & Biomolecular Chemistry, 3, (22), 4153-4157. (doi: 10.1039/b511706b)
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Official URL: http://dx.doi.org/10.1039/b511706b
Description/Abstract
We examined the stability and kinetics of folding of the Oxytricha telomeric repeat sequence (G4T4)4. Fluorescence melting experiments show that this intramolecular quadruplex, which is more stable in potassium- than sodium-containing buffers, shows considerable hysteresis between the melting and annealing profiles, even when heated at a rate of 0.05 °C min–1. Quantitative analysis of this hysteresis, together with temperature-jump relaxation experiments show that the dissociation is exceptionally slow with a half-life of about 10 years at 37 °C in the presence of 50 mM K+. The association reaction has a half-life of a few seconds at 37 °C, but becomes slower at elevated temperatures consistent with the suggestion that association occurs by a nucleation-zipper mechanism.
| Item Type: | Article |
|---|---|
| ISSN: | 1477-0520 (print) |
| Uncontrolled Keywords: | dna quadruplexes, g-quartet, crystal-structure, nucleic-acids, g-tetraplex, stability, potassium, loop, oligonucleotides, parallel |
| Related URLs: | http://www.ncbi.nlm.nih.gov/en...med_docsum http://dx.doi.org/10.1039/b511706b |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH426 Genetics |
| Divisions: | University Structure - Pre August 2011 > School of Chemistry |
| ePrint ID: | 20746 |
| URI: | http://eprints.soton.ac.uk/id/eprint/20746 |
| Deposited On: | 02 Mar 2006 |
| Last Modified: | 01 Jun 2011 02:51 |
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