A simple setup for the study of microvolume frozen samples using Raman spectroscopy
Kriek, Marco, Neylon, Cameron, Roach, Peter L., Clark, Ian P. and Parker, Anthony W. (2005) A simple setup for the study of microvolume frozen samples using Raman spectroscopy. Review of Scientific Instruments, 76, (10) (doi: 10.1063/1.2054486).
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Description/Abstract
A method for obtaining resonance Raman spectra from microvolume (2-5 mu l) frozen samples is described. The procedure involves trapping and freezing a microdroplet in a specially designed sample holder using a cryostream. The method makes it possible to prepare numerous low-volume air-sensitive samples in a suitable environment for spectroscopic studies. We have demonstrated the potential of the setup measuring the resonance Raman spectra of 4-thiouracil and horse heart myoglobin.
| Item Type: | Article |
|---|---|
| Related URLs: | |
| Keywords: | resonance raman, vibrational-modes, heme-proteins, myoglobin, spectra, ferredoxin, crystals, binding, spin |
| Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
| Divisions: | University Structure - Pre August 2011 > School of Chemistry |
| Item ID: | 20840 |
| Date Deposited: | 02 Mar 2006 |
| Last Modified: | 01 Jun 2011 03:58 |
| Contributors: | Kriek, Marco (Author) Neylon, Cameron (Author) Roach, Peter L. (Author) Clark, Ian P. (Author) Parker, Anthony W. (Author) |
| Date: | 1 October 2005 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/20840 |
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