Adsorption of prion and tissue proteins to surgical stainless steel surfaces and the efficacy of decontamination following dry and wet storage conditions
Secker, T.J., Hervé, R. and Keevil, C.W. (2011) Adsorption of prion and tissue proteins to surgical stainless steel surfaces and the efficacy of decontamination following dry and wet storage conditions. Journal of Hospital Infection, 78, (4), 251-255. (doi:10.1016/j.jhin.2011.03.021).
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Iatrogenic transmission of the infectious prion protein (PrP(Sc)) is a potential threat due to its resistance to many chemical and enzymatic decontamination protocols and its strong adhesive properties to stainless steel. The conditions in which surgical instruments are handled during and after surgery may affect the level of tissue protein, prion attachment and the efficacy of subsequent decontamination regimes. This study investigated the adhesion of tissue protein and prion-associated amyloid to surgical stainless steel with respect to time and various storage conditions, and the subsequent outcome on the efficacy of enzymatic cleaning chemistries. Surfaces were contaminated with ME7-infected brain homogenate and left to dry between 0 and 120 min at room temperature or 24 h, in dry or moist conditions. Residual contamination before and after cleaning was visualised using sensitive fluorescent staining and episcopic differential interference contrast/epifluorescence microscopy. Longer drying times increased both protein and prion amyloid adsorption and affected the efficacy of the cleaning chemistries tested. A moist environment post-contamination significantly reduced the attachment of both protein and prion amyloid to the surgical stainless steel surface. Maintaining moist conditions could potentially improve the subsequent decontamination of reusable surgical instruments, also reducing process time and cost
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculty of Natural and Environmental Sciences > Biological Sciences
|Date Deposited:||27 Jan 2012 11:21|
|Last Modified:||26 Apr 2013 01:26|
|Contributors:||Secker, T.J. (Author)
Hervé, R. (Author)
Keevil, C.W. (Author)
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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