Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry
463-470
Azevedo, N.F.
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Jardim, T.
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Almeida, C.
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Cerqueira, L.
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Almeida, A.J.
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Rodrigues, F.
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Keevil, C W.
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Vieira, M.J.
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October 2011
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Jardim, T.
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Almeida, C.
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Cerqueira, L.
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Almeida, A.J.
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Rodrigues, F.
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Keevil, C W.
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Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Azevedo, N.F., Jardim, T., Almeida, C., Cerqueira, L., Almeida, A.J., Rodrigues, F., Keevil, C W. and Vieira, M.J.
(2011)
Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples.
Antonie van Leeuwenhoek, 100 (3), .
(doi:10.1007/s10482-011-9595-9).
(PMID:21638111)
Abstract
Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry
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e-pub ahead of print date: 3 June 2011
Published date: October 2011
Organisations:
Centre for Biological Sciences
Identifiers
Local EPrints ID: 209287
URI: http://eprints.soton.ac.uk/id/eprint/209287
ISSN: 0003-6072
PURE UUID: e330d74e-8fce-4ea1-b5a2-f87ae506dc10
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Date deposited: 27 Jan 2012 11:28
Last modified: 15 Mar 2024 03:12
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Contributors
Author:
N.F. Azevedo
Author:
T. Jardim
Author:
C. Almeida
Author:
L. Cerqueira
Author:
A.J. Almeida
Author:
F. Rodrigues
Author:
M.J. Vieira
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