Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
Azevedo, N.F., Jardim, T., Almeida, C., Cerqueira, L., Almeida, A.J., Rodrigues, F., Keevil, C W. and Vieira, M.J. (2011) Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples. Antonie van Leeuwenhoek, 100, (3), 463-470. (doi:10.1007/s10482-011-9595-9). (PMID:21638111).
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Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry
|Digital Object Identifier (DOI):||doi:10.1007/s10482-011-9595-9|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculty of Natural and Environmental Sciences > Biological Sciences
|Date Deposited:||27 Jan 2012 11:28|
|Last Modified:||31 Mar 2016 13:49|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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