Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
Almeida, C., Azevedo, N.F., Iversen, C., Fanning, S., Keevil, C.W. and Vieira, M.J. (2009) Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula. Applied and Environmental Microbiology, 75, (9), 2925-2930. (doi:10.1128/AEM.02470-08). (PMID:19270117).
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Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
|Digital Object Identifier (DOI):||doi:10.1128/AEM.02470-08|
|Subjects:||Q Science > QR Microbiology
R Medicine > RJ Pediatrics
|Divisions:||Faculty of Natural and Environmental Sciences > Biological Sciences
|Date Deposited:||31 Jan 2012 12:31|
|Last Modified:||27 Mar 2014 19:50|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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