Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish

Nagayama, Toshiki, Kimura, Ken-Ichi, Araki, Makoto, Aonuma, Hitoshi and Newland, Philip L. (2004) Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish. Journal of Comparative Neurology, 474, (1), 123-135. (doi:10.1002/cne.20124).


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Original Publication URL: http://dx.doi.org/10.1002/cne.20124


Using an antiserum directed against glutamate, we have analyzed the distribution of
glutamate-like immunoreactive neurons in the terminal abdominal ganglion of the crayfish
Procambarus clarkii. Approximately 160 central neurons (157 8; mean SEM, n 8) showed
positive glutamate-like immunoreactivity, which represents approximately 25% of the total
number of neurons in the terminal ganglion. Using a combination of intracellular staining with
the marker Lucifer yellow and immunocytochemical staining has shown that most excitatory
motor neurons are glutamatergic and that glutamate acts as an excitatory transmitter at
peripheral neuromuscular junctions. Seven of 10 identified spiking local interneurons and only 2
of 19 identified ascending interneurons, showed positive immunoreactivity. Our observation that
inhibitory spiking interneurons were immunopositive, whereas excitatory ascending interneurons
were immunonegative, indicates that glutamate is likely to act as an inhibitory neurotransmitter
within the central nervous system. Local pressure injection of L-glutamate into the
neuropil of the ganglion caused a hyperpolarization of the membrane potentials of many interneurons.
-Aminobutyric acid (GABA)ergic posterolateral nonspiking interneurons and the bilateral
nonspiking interneuron LDS showed no glutamate-like immunoreactivity, whereas non-
GABAergic anterolateral III nonspiking interneurons showed glutamate-like immunoreactivity.
Thus, not only GABA but also glutamate are used in parallel as inhibitory neurotransmitters at
central synapses.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1002/cne.20124
ISSNs: 0021-9967 (print)
Related URLs:
Keywords: glutamate, double labeling, identified neurons, gaba, inhibitory transmitter
Subjects: Q Science > QR Microbiology > QR180 Immunology
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 24218
Accepted Date and Publication Date:
14 June 2004Published
3 October 2003Submitted
Date Deposited: 28 Mar 2006
Last Modified: 31 Mar 2016 11:45
URI: http://eprints.soton.ac.uk/id/eprint/24218

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