A dedicated translation factor controls the synthesis of the global regulator Fis

Owens, R., Pritchard, G., Skipp, P., Hodey, M., Connell, S.R., Nierhaus, K.H. and O'Connor, D. (2004) A dedicated translation factor controls the synthesis of the global regulator Fis. The EMBO Journal, 23, 3375-3385. (doi:10.1038/sj.emboj.7600343).


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BipA is a highly conserved protein with global regulatory
properties in Escherichia coli. We show here that it functions
as a translation factor that is required specifically for
the expression of the transcriptional modulator Fis. BipA
binds to ribosomes at a site that coincides with that of
elongation factor G and has a GTPase activity that is
sensitive to high GDP:GTP ratios and stimulated by 70S
ribosomes programmed with mRNA and aminoacylated
tRNAs. The growth rate-dependent induction of BipA
allows the efficient expression of Fis, thereby modulating
a range of downstream processes, including DNA metabolism
and type III secretion. We propose a model in which
BipA destabilizes unusually strong interactions between
the 50 untranslated region of fis mRNA and the ribosome.
Since BipA spans phylogenetic domains, transcript-selective
translational control for the ‘fast-track’ expression of
specific mRNAs may have wider significance.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1038/sj.emboj.7600343
ISSNs: 0261-4189 (print)
Related URLs:
Keywords: proteins; cellular metabolism Keywords: Fis; GTPase; regulation; ribosome; translation
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 24222
Accepted Date and Publication Date:
5 August 2004Published
Date Deposited: 28 Mar 2006
Last Modified: 31 Mar 2016 11:45
URI: http://eprints.soton.ac.uk/id/eprint/24222

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