Non-invasive amino acid turnover predicts human embryo developmental capacity
Houghton, Franchesca D., Hawkhead, Judith A., Humpherson, Peter G., Hogg, Jan E., Balen, Adam H., Rutherford, Anthony J. and Leese, Henry J. (2002) Non-invasive amino acid turnover predicts human embryo developmental capacity. Human Reproduction, 17, (4), 999-1005. (doi:10.1093/humrep/17.4.999).
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Background: IVF is limited by low success rates and a confounding high multiple birth rate contributing to prematurity, increased neonatal mortality and child handicap. These problems could be overcome if single embryos of known developmental competence could be selected for transfer on day 2/3 of development, but current methods, which rely on morphological appearance, are poor predictors of viability.
Methods: We have measured non-invasively the depletion/appearance (i.e. turnover) of a physiological mixture of 18 amino acids by single human embryos during in-vitro culture using high performance liquid chromatography.
Results: From the time of transfer (day 2/3), embryos with future competence to develop to the blastocyst stage (day 5/6) exhibit amino acid flux patterns distinct from those of embryos with similar morphological appearance which arrest. Significantly, the profiles of Ala, Arg, Gln, Met and Asn flux predict blastocyst potentiality at >95%. The amino acid most consistently depleted throughout development by those embryos which form blastocysts was leucine. Of the amino acids which were produced, the most striking was alanine, which appeared in increasing amounts throughout development.
Conclusions: Non-invasive amino acid profiling has the potential to select developmentally competent single embryos for transfer, thereby increasing the success rate and eliminating multiple births in IVF.
|Keywords:||amino acids, developmental potential, human preimplantation embryo culture, IVF|
|Subjects:||R Medicine > RG Gynecology and obstetrics
Q Science > QH Natural history > QH426 Genetics
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Human Genetics
|Date Deposited:||05 Apr 2006|
|Last Modified:||06 Aug 2015 02:24|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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