PIRA PCR designer for restriction analysis of single nucleotide polymorphisms
PIRA PCR designer for restriction analysis of single nucleotide polymorphisms
Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.
Availability: The computer program, with related descriptions, is available at http://cedar.genetics.soton.ac.uk/public_html/primer2.html
838-839
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
May 2001
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
Ke, Xiayi, Collins, Andrew and Ye, Shu
(2001)
PIRA PCR designer for restriction analysis of single nucleotide polymorphisms.
Bioinformatics, 17 (9), .
(doi:10.1093/bioinformatics/17.9.838).
Abstract
Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.
Availability: The computer program, with related descriptions, is available at http://cedar.genetics.soton.ac.uk/public_html/primer2.html
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Published date: May 2001
Identifiers
Local EPrints ID: 24798
URI: http://eprints.soton.ac.uk/id/eprint/24798
ISSN: 1367-4803
PURE UUID: 82008d72-dc67-4276-92c9-3d3ee31362a4
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Date deposited: 05 Apr 2006
Last modified: 16 Mar 2024 02:42
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Author:
Xiayi Ke
Author:
Shu Ye
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