Beta cell differentiation during early human pancreas development


Piper, K., Brickwood, S., Turnpenny, L.W., Cameron, I.T., Ball, S.G., Wilson, D.I. and Hanley, N.A. (2004) Beta cell differentiation during early human pancreas development. Journal of Endocrinology, 181, (1), 11-23. (doi:10.1677/joe.0.1810011).

Download

Full text not available from this repository.

Original Publication URL: http://dx.doi.org/10.1677/joe.0.1810011

Description/Abstract

Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c., human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy.

Item Type: Article
ISSNs: 0022-0795 (print)
Related URLs:
Subjects: R Medicine > RG Gynecology and obstetrics
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Medicine > Human Genetics
ePrint ID: 24905
Date Deposited: 04 Apr 2006
Last Modified: 27 Mar 2014 18:14
Contact Email Address: n.a.hanley@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/24905

Actions (login required)

View Item View Item