Typing dinucleotide repeat loci using microplate array diagonal gel electrophoresis: proof of principle
Rodriguez, Santiago, Chen, Xiao-he and Day, Ian N.M. (2004) Typing dinucleotide repeat loci using microplate array diagonal gel electrophoresis: proof of principle. Electrophoresis, 25, (7-8), 975-979. (doi:10.1002/elps.200305801).
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Description/Abstract
Polymorphic dinucleotide repeat loci (microsatellite markers) are found in varying abundance throughout the genomes of most organisms. They have been extensively used for genetic studies, but conventional techniques used for their genotyping require sophisticated equipment. Microplate array diagonal gel electrophoresis (MADGE) has previously been extended to economical high-throughput genotyping of trinucleotide and tetranucleotide microsatellite amplicons. However, the capability of this technique to resolve the alleles of dinucleotide repeat loci has not been explored previously. Here we show that a modified microsatellite-MADGE approach can provide sufficient resolution for dinucleotide repeat typing. This enables economical and convenient set up for analysis of single markers in many samples in parallel, suitable, for example, for population association studies.
| Item Type: | Article |
|---|---|
| ISSNs: | 0173-0835 (print) |
| Related URLs: | |
| Keywords: | dinucleotide repeat loci, high-throughput genotyping, microplate array diagonal gel electrophoresis |
| Subjects: | R Medicine |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Human Genetics |
| Item ID: | 24927 |
| Date Deposited: | 05 Apr 2006 |
| Last Modified: | 01 Jun 2011 11:15 |
| Contributors: | Rodriguez, Santiago (Author) Chen, Xiao-he (Author) Day, Ian N.M. (Author) |
| Date: | April 2004 |
| Status: | Published |
| Contact Email Address: | santi@soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/24927 |
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