Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF
Thurston, L.M., Norgate, D.P., Jonas, K.C., Gregory, L., Wood, P.J., Cooke, B.A. and Michael, A.E. (2003) Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF. Human Reproduction, 18, (8), 1603-1612. (doi:10.1093/humrep/deg322). (PMID:12871869).
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Background: Follicular fluid (FF) contains compounds that can modulate NADP+-dependent oxidation of cortisol by type 1 11?-hydroxysteroid dehydrogenase (11?HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11?HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles.
Methods: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP+-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer.
Results: Levels of the hydrophilic ovarian 11?HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11?HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11?HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 ± 0.3 versus 8.5 ± 0.2, respectively; P < 0.0001).
Conclusions: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11?HSD.
|Digital Object Identifier (DOI):||doi:10.1093/humrep/deg322|
|Keywords:||biology, ovary, molecular biology, humans, adult, activity,non-u.s.gov't, london, in vitro, pregnancy, animals,11-beta-hydroxysteroid dehydrogenases, oxidation-reduction, methods|
|Subjects:||Q Science > QH Natural history > QH301 Biology
R Medicine > RG Gynecology and obstetrics
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Human Genetics
|Date Deposited:||06 Apr 2006|
|Last Modified:||31 May 2016 10:01|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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