Solid-phase extraction in clinical biochemistry
Walker, Valerie and Mills, Graham A. (2002) Solid-phase extraction in clinical biochemistry. Annals of Clinical Biochemistry, 39, (5), 464-477. (doi:10.1258/000456302320314476).
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Description/Abstract
In order to measure low concentrations of analytes in plasma and urine, it is often necessary to extract and concentrate them. With solid-phase extraction (SPE), this is achieved by partitioning the analytes between a solid and a liquid or headspace vapour. A wide range of high-quality materials is now available to do this, offering a variety of separation modes for different applications. These include partitioning using reversed-phase, normal-phase, ion-exchange, restricted-access and immunoaffinity sorbents or molecularly imprinted polymers and, increasingly, combinations of these processes. Solid-phase microextraction was introduced to analyse volatile and semi-volatile compounds. The range of sampling formats has expanded from simple packed syringes to cartridges, disks, SPE pipette tips and 96-well plates. These developments have facilitated automated off- and on-line sample processing. The basic principles of SPE and the recent innovations are reviewed here. This is a technological growth area. Some of the developments are finding application in clinical toxicology. However, they could also be of wider value in clinical chemistry - for example, for analyses of volatile and non-volatile metabolites, peptides, radioactive elements and trace metal speciation.
| Item Type: | Article |
|---|---|
| ISSNs: | 0004-5632 (print) |
| Related URLs: | |
| Subjects: | R Medicine Q Science > QR Microbiology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Human Genetics |
| Item ID: | 25024 |
| Date Deposited: | 06 Apr 2006 |
| Last Modified: | 01 Jun 2011 13:49 |
| Contributors: | Walker, Valerie (Author) Mills, Graham A. (Author) |
| Date: | 1 September 2002 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/25024 |
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