Selective synthesis of bone morphogenetic proteins-1, -3, -4 and bone sialoprotein may be important for osteoinduction by Saos-2 cells
Anderson, H. Clarke, Reynolds, Paul R., Hsu, Howard H.T., Missana, Lilliana, Masuhara, Kensaku, Moylan, Paul E. and Roach, Helmtrude I. (2002) Selective synthesis of bone morphogenetic proteins-1, -3, -4 and bone sialoprotein may be important for osteoinduction by Saos-2 cells. Journal of Bone and Mineral Metabolism, 20, (2), 73-82. (doi:10.1007/s007740200010).
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Description/Abstract
An ability to induce new bone formation at a required site would represent a considerable advance in bone repair and tissue engineering. It has been shown that the healing of critical-size bone defects in rats can be augmented by extracts of Saos-2 cells. These human osteosarcoma cells uniquely contain a bone-inducing activity, whereas other human osteosarcoma cells, e.g., U-2 OS cells, cannot replicate the osteoinductive capacity. To understand the necessary components of the Saos-2 bone-inducing activity, this study compared osteoinductive Saos-2 cells with non-osteoinductive U-2 OS cells with respect to the synthesis of bone morphogenetic proteins (BMPs)-1, -2, -3, -4, -5, -6, and -7 and the non-collagenous matrix proteins bone sialoprotein (BSP), osteonectin (ON), osteopontin (OPN), and osteocalcin (OC). The main differences were abundant synthesis of BMP-1/tolloid, BMP-3, -4, and BSP by Saos-2 cells, but absence or reduced synthesis in U-2 OS cells. BMP-2 and -7 were present in low amounts in both cell types, while BMP-5 and -6 were more abundant in U-2 OS cells, suggesting that these BMPs were of lesser importance for the osteoinductivity of Saos-2 cells. However, a relatively high expression of BMP-3 and -4, together with BMP-1/tolloid, may be important for the osteoinductive capacity of Saos-2 cells. The inability of U2-OS cells to induce bone, despite expressing most of the BMPs, may be due to an insufficiency of tolloid, BMP-3 or -4, BSP, and/or other unknown factors. A better understanding of the necessary components of the Saos-2 cell bone-inducing agent may, in future, lead to clinically useful Saos-2 cell products for bone repair and tissue engineering.
| Item Type: | Article |
|---|---|
| ISSNs: | 0914-8779 (print) |
| Related URLs: | |
| Keywords: | bone morphogenetic proteins, bone sialopro-tein, osteoinduction, tolloid, saos-2 cells |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology Q Science > QP Physiology Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease |
| Item ID: | 25198 |
| Date Deposited: | 07 Apr 2006 |
| Last Modified: | 01 Jun 2011 13:49 |
| Contributors: | Anderson, H. Clarke (Author) Reynolds, Paul R. (Author) Hsu, Howard H.T. (Author) Missana, Lilliana (Author) Masuhara, Kensaku (Author) Moylan, Paul E. (Author) Roach, Helmtrude I. (Author) |
| Date: | March 2002 |
| Status: | Published |
| Contact Email Address: | hr@soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/25198 |
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