Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube
Davies, S., Richardson, M.C., Anthony, F.W., Mukhtar, D.D. and Cameron, I.T. (2004) Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube. Molecular Human Reproduction, 10, (12), 935-939. (doi:10.1093/molehr/gah124).
The Fallopian tube provides the environment for early embryo growth, a process which is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bioavailability of tubal IGFs is modulated by locally produced IGF-binding protein (IGFBP-1) is not clear. An explant culture system from human Fallopian tube mucosa was, therefore, developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. Thus, oviduct-specific glycoprotein production, a major product of the oviduct in vivo, continued to be made via an estrogen-sensitive pathway in the culture. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT–PCR and IGFBP-1 protein was measured by enzyme-linked immunosorbent assay. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein levels. A dose-range of progesterone revealed an incremental inhibitory effect of progesterone on IGFBP-1 output (maximal effect, 25–50 nmol/l), consistent with physiological inhibition of this process during the luteal phase. We suggest that progesterone might, therefore, play a role in controlling the bioavailability of IGFs to the embryo during early development within the Fallopian tube.
|Keywords:||fallopian tube, IGFBP-1, oviduct-specific glycoprotein, progesterone|
|Subjects:||R Medicine > RG Gynecology and obstetrics|
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
|Date Deposited:||07 Apr 2006|
|Last Modified:||28 Jun 2012 10:06|
|Contributors:||Davies, S. (Author)
Richardson, M.C. (Author)
Anthony, F.W. (Author)
Mukhtar, D.D. (Author)
Cameron, I.T. (Author)
|Contact Email Address:||email@example.com|
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