Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube


Davies, S., Richardson, M.C., Anthony, F.W., Mukhtar, D.D. and Cameron, I.T. (2004) Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube. Molecular Human Reproduction, 10, (12), 935-939. (doi:10.1093/molehr/gah124).

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Original Publication URL: http://dx.doi.org/10.1093/molehr/gah124

Description/Abstract

The Fallopian tube provides the environment for early embryo growth, a process which is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bioavailability of tubal IGFs is modulated by locally produced IGF-binding protein (IGFBP-1) is not clear. An explant culture system from human Fallopian tube mucosa was, therefore, developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. Thus, oviduct-specific glycoprotein production, a major product of the oviduct in vivo, continued to be made via an estrogen-sensitive pathway in the culture. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT–PCR and IGFBP-1 protein was measured by enzyme-linked immunosorbent assay. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein levels. A dose-range of progesterone revealed an incremental inhibitory effect of progesterone on IGFBP-1 output (maximal effect, 25–50 nmol/l), consistent with physiological inhibition of this process during the luteal phase. We suggest that progesterone might, therefore, play a role in controlling the bioavailability of IGFs to the embryo during early development within the Fallopian tube.

Item Type: Article
ISSNs: 1360-9947 (print)
Related URLs:
Keywords: fallopian tube, IGFBP-1, oviduct-specific glycoprotein, progesterone
Subjects: R Medicine > RG Gynecology and obstetrics
Divisions: University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
ePrint ID: 25394
Date Deposited: 07 Apr 2006
Last Modified: 27 Mar 2014 18:14
Contact Email Address: mcr2@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/25394

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