The University of Southampton
University of Southampton Institutional Repository

Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period

Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period
Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period
Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 ± 2.2 years, body mass index 22.1 ± 2.3 kg/m2) were fed a mixed meal containing 30 g fish oil and 600 mg [1-13C]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apolipoprotein B-100. The fatty acid composition of lipoproteins was analyzed by gas chromatography/mass spectrometry. [1-13C]palmitic acid started to appear in VLDL TG 3 h after meal intake, and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Approximately 20% of dietary fatty acids entered the VLDL TG pool 6 h after meal intake. DHA was clearly overincorporated into this pool compared with [1-13C]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the nonesterified fatty acid pool.

In summary, the contribution of dietary fatty acids to early postprandial VLDL TG is substantial. The role of DHA in VLDL TG production will require further investigation.

chylomicron, apolipoprotein b, immunoaffinity chromatography, triacylglycerol, n-3 polyunsaturated fatty acids, palmitic acid, stable isotopes
0022-2275
2065-2072
Heath, Richard B.
485b73bf-cfac-41b6-a3b3-849fc2f62007
Karpe, Fredrik
05abcb32-83b7-44eb-ab12-7c360f4f9c12
Milne, Ross W.
7a87dfad-d47a-4fd7-9ddd-b0e2321631d4
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Frayn, Keith N.
43ece647-8996-45ba-a004-2c921f2d3ca4
Heath, Richard B.
485b73bf-cfac-41b6-a3b3-849fc2f62007
Karpe, Fredrik
05abcb32-83b7-44eb-ab12-7c360f4f9c12
Milne, Ross W.
7a87dfad-d47a-4fd7-9ddd-b0e2321631d4
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Frayn, Keith N.
43ece647-8996-45ba-a004-2c921f2d3ca4

Heath, Richard B., Karpe, Fredrik, Milne, Ross W., Burdge, Graham C., Wootton, Stephen A. and Frayn, Keith N. (2003) Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period. Journal of Lipid Research, 44 (11), 2065-2072. (doi:10.1194/jlr.M300167-JLR200).

Record type: Article

Abstract

Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 ± 2.2 years, body mass index 22.1 ± 2.3 kg/m2) were fed a mixed meal containing 30 g fish oil and 600 mg [1-13C]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apolipoprotein B-100. The fatty acid composition of lipoproteins was analyzed by gas chromatography/mass spectrometry. [1-13C]palmitic acid started to appear in VLDL TG 3 h after meal intake, and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Approximately 20% of dietary fatty acids entered the VLDL TG pool 6 h after meal intake. DHA was clearly overincorporated into this pool compared with [1-13C]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the nonesterified fatty acid pool.

In summary, the contribution of dietary fatty acids to early postprandial VLDL TG is substantial. The role of DHA in VLDL TG production will require further investigation.

This record has no associated files available for download.

More information

Published date: 2003
Keywords: chylomicron, apolipoprotein b, immunoaffinity chromatography, triacylglycerol, n-3 polyunsaturated fatty acids, palmitic acid, stable isotopes

Identifiers

Local EPrints ID: 25604
URI: http://eprints.soton.ac.uk/id/eprint/25604
ISSN: 0022-2275
PURE UUID: bfc874f6-7838-42e2-b29c-256922dfcaff
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 19 Apr 2006
Last modified: 15 Mar 2024 07:03

Export record

Altmetrics

Contributors

Author: Richard B. Heath
Author: Fredrik Karpe
Author: Ross W. Milne
Author: Keith N. Frayn

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×