Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens
Miles, Elizabeth A., Bakewell, Lucy and Calder, Philip C. (2003) Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens. Cytokine, 21, (2), 74-83. (doi:10.1016/S1043-4666(02)00502-1).
Full text not available from this repository.
A whole blood culture technique was used to establish conditions for stimulating the production of cytokines by cord blood lymphocytes. The cultures were stimulated with mitogens (concanavalin A and phytohaemagglutinin) and allergens (beta-lactoglobulin (BLG), ovalbumin (OVA) and house dust mite (Der p 1)) at a range of concentrations. Interleukin (IL-) 2, IL-4, IL-10, IL-13 and interferon-gamma (IFN-γ) concentrations were assayed in the supernatants at 24, 48 and 72 h. Stimulation with mitogens but not allergens induced increases in IL-2 and IL-13 concentrations. IFN-γ was strongly induced by mitogenic stimulation: maximal responses were seen at 48 h. Stimulation with the allergens also induced an increase in IFN-γ concentration which was maximal for 100 μg/ml of BLG and OVA. Der p 1 induced the highest IFN-γ production among the allergens. IL-4 concentrations were increased in mitogen and Der p 1 stimulated cultures. This was maximal at 48 and 24 h, respectively. IL-10 was induced with mitogen and allergen stimulation. Thus, this study has established conditions for assessing production of lymphocyte-derived cytokines in a simple whole umbilical cord blood culture system.
|Keywords:||allergen, cord blood, cytokine, interleukin, whole blood|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
|Date Deposited:||20 Apr 2006|
|Last Modified:||01 Jun 2011 04:10|
|Contributors:||Miles, Elizabeth A. (Author)
Bakewell, Lucy (Author)
Calder, Philip C. (Author)
|Contact Email Address:||email@example.com|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
Actions (login required)