Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens
Miles, Elizabeth A., Bakewell, Lucy and Calder, Philip C. (2003) Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens. Cytokine, 21, (2), 74-83. (doi:10.1016/S1043-4666(02)00502-1).
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Description/Abstract
A whole blood culture technique was used to establish conditions for stimulating the production of cytokines by cord blood lymphocytes. The cultures were stimulated with mitogens (concanavalin A and phytohaemagglutinin) and allergens (beta-lactoglobulin (BLG), ovalbumin (OVA) and house dust mite (Der p 1)) at a range of concentrations. Interleukin (IL-) 2, IL-4, IL-10, IL-13 and interferon-gamma (IFN-γ) concentrations were assayed in the supernatants at 24, 48 and 72 h. Stimulation with mitogens but not allergens induced increases in IL-2 and IL-13 concentrations. IFN-γ was strongly induced by mitogenic stimulation: maximal responses were seen at 48 h. Stimulation with the allergens also induced an increase in IFN-γ concentration which was maximal for 100 μg/ml of BLG and OVA. Der p 1 induced the highest IFN-γ production among the allergens. IL-4 concentrations were increased in mitogen and Der p 1 stimulated cultures. This was maximal at 48 and 24 h, respectively. IL-10 was induced with mitogen and allergen stimulation. Thus, this study has established conditions for assessing production of lymphocyte-derived cytokines in a simple whole umbilical cord blood culture system.
| Item Type: | Article |
|---|---|
| ISSNs: | 1043-4666 (print) |
| Related URLs: | |
| Keywords: | allergen, cord blood, cytokine, interleukin, whole blood |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease |
| Item ID: | 25811 |
| Date Deposited: | 20 Apr 2006 |
| Last Modified: | 01 Jun 2011 04:10 |
| Contributors: | Miles, Elizabeth A. (Author) Bakewell, Lucy (Author) Calder, Philip C. (Author) |
| Date: | 2003 |
| Status: | Published |
| Contact Email Address: | eam@soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/25811 |
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