Effects of polyphenols on human Th1 and Th2 cytokine production

Miles, E.A., Zoubouli, P. and Calder, P.C. (2005) Effects of polyphenols on human Th1 and Th2 cytokine production. Clinical Nutrition, 24 (5), 780-784. (doi:10.1016/j.clnu.2005.04.001).

Abstract

Background Numerous phenolic compounds are consumed in the diet in a range of foods. There are very few studies of the effects of these compounds on the production of lymphocyte-derived cytokines.
Aim of the study To investigate the effects of five phenolic compounds on cytokine production by cultured human lymphocytes.
Methods Human whole blood cultures were stimulated with the T cell stimulant concanavalin A for 48 h in the presence of phenolic compounds (vanillic acid, syringic acid, kaempferol, oleuropein and tyrosol) at concentrations up to 10?4 M. Interleukin (IL)-2, IL-4 and interferon-? (IFN-?) concentrations were measured in the culture supernatants by ELISA.
Results IFN-? concentration was significantly lower in cultures containing 10?4 M kaempferol than in cultures with kaempferol at 10?7, 10?6and 10?5 M or without kaempferol. The other phenolic compounds did not affect IFN-? concentration and none of the phenolics tested affected IL-2 or IL-4 concentrations.
Conclusions Some, but not all, phenolic compounds can decrease IFN-? production by stimulated human whole blood cultures.

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Contributors

Author: E.A. Miles

Author: P.C. Calder

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