Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies
Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies
Bone tissue loss as a consequence of the natural aging process or as a result of trauma and degenerative disease has led to the need for procedures to generate cartilage and bone for a variety of orthopedic applications. The ability to transfer genes into multipotential mesenchymal stem cells, while still in its infancy, offers considerable therapeutic hope in a variety of musculoskeletal disorders. However, the choice of gene delivery method is key. This review examines the various techniques and methods currently available to enable gene transfer into a target population from viral methods (transduction) to nonviral (transfection) methods and the limitations associated with each method. The potential applications and current understanding of each method are presented. Given the demographic challenge of an aging population, the ultimate goal remains the development of simple, safe, and reproducible strategies for gene delivery that will address the pressing orthopedic clinical imperatives of many.
295-307
Partridge, Kris A.
034d205b-2718-4503-b2e6-85d2dee4af80
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
January 2004
Partridge, Kris A.
034d205b-2718-4503-b2e6-85d2dee4af80
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Partridge, Kris A. and Oreffo, Richard O. C.
(2004)
Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies.
Tissue Engineering, 10 (1-2), .
Abstract
Bone tissue loss as a consequence of the natural aging process or as a result of trauma and degenerative disease has led to the need for procedures to generate cartilage and bone for a variety of orthopedic applications. The ability to transfer genes into multipotential mesenchymal stem cells, while still in its infancy, offers considerable therapeutic hope in a variety of musculoskeletal disorders. However, the choice of gene delivery method is key. This review examines the various techniques and methods currently available to enable gene transfer into a target population from viral methods (transduction) to nonviral (transfection) methods and the limitations associated with each method. The potential applications and current understanding of each method are presented. Given the demographic challenge of an aging population, the ultimate goal remains the development of simple, safe, and reproducible strategies for gene delivery that will address the pressing orthopedic clinical imperatives of many.
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Published date: January 2004
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Local EPrints ID: 25891
URI: http://eprints.soton.ac.uk/id/eprint/25891
ISSN: 1076-3279
PURE UUID: 4b1a190d-c49d-489f-bcc2-96fce41f751b
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Date deposited: 20 Apr 2006
Last modified: 09 Jan 2022 03:02
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Author:
Kris A. Partridge
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