Conductivity in Jurkat cell suspension after ultrashort electric pulsing


Chen, G, Chen, N, Garner, A L, Kolb, J, Swanson, R J, Beebe, S, Joshi, R P and Schoenbach, K H (2004) Conductivity in Jurkat cell suspension after ultrashort electric pulsing. At 3rd International workshop on biological effect of EMFs, Kos, Greece, 04 - 08 Oct 2004. , 56-65.

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Description/Abstract

Ultrashort electric pulses applied to similar cell lines such as Jurkat and HL-60 cells can produce markedly different results , which have been documented extensively over the last few years. We now report changes in electrical conductivity of Jurkat cells subjected to traditional electroporation pulses (50 ms pulse length) and ultrashort pulses (10 ns pulse length) using time domain dielectric spectroscopy (TDS). A single 10 ns, 150 kV/cm pulse did not noticeably alter suspension conductivity while a 50 ms, 2.12 kV/cm pulse with the same energy caused an appreciable conductivity rise. These results support the hypothesis that electroporation pulses primarily interact with the cell membrane and cause conductivity rises due to ion transport from the cell to the external media, while pulses with nanosecond duration primarily interact with the membranes of intracellular organelles. However, multiple ultrashort pulses have a cumulative effect on the plasma membrane, with five pulses causing a gradual rise in conductivity up to ten minutes post-pulsing.

Item Type: Conference or Workshop Item (Speech)
Additional Information: Event Dates: Oct 4--8, 2004
ISBNs: 9602331518
Keywords: Jurkat cell, electrical conductivity, ultrashort electric pulse
Divisions: Faculty of Physical Sciences and Engineering > Electronics and Computer Science
Faculty of Physical Sciences and Engineering > Electronics and Computer Science > EEE
ePrint ID: 260168
Date Deposited: 01 Dec 2004
Last Modified: 27 Mar 2014 20:02
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/260168

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