Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo


Thomas, F.C., Sheth, B., Eckert, J.J., Bazzoni, G., Dejana, E. and Fleming, T.P. (2004) Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo. Journal of Cell Science, 117, (23), 5599-5608. (doi: 10.1242/jcs.01424).

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Original Publication URL: http://dx.doi.org/10.1242/jcs.01424

Description/Abstract

We have investigated the contribution of the tight junction (TJ) transmembrane protein junction-adhesion-molecule 1 (JAM-1) to trophectoderm epithelial differentiation in the mouse embryo. JAM-1-encoding mRNA is expressed early from the embryonic genome and is detectable as protein from the eight-cell stage. Immunofluorescence confocal analysis of staged embryos and synchronized cell clusters revealed JAM-1 recruitment to cell contact sites occurred predominantly during the first hour after division to the eight-cell stage, earlier than any other TJ protein analysed to date in this model and before E-cadherin adhesion and cell polarization. During embryo compaction later in the fourth cell cycle, JAM-1 localized transiently yet precisely to the apical microvillous pole, where protein kinase C (PKC) and PKC are also found, indicating a role in cell surface reorganization and polarization. Subsequently, in morulae and blastocysts, JAM-1 is distributed ubiquitously at cell contact sites within the embryo but is concentrated within the trophectoderm apicolateral junctional complex, a pattern resembling that of E-cadherin and nectin-2. However, treatment of embryos with anti-JAM-1-neutralizing antibodies indicated that JAM-1 did not contribute to global embryo compaction and adhesion but rather regulated the timing of blastocoel cavity formation dependent upon establishment of the trophectoderm TJ paracellular seal.

Item Type: Article
Additional Information: All the work for this paper took place within my laboratory and funded by my grants (Tom Fleming)
ISSNs: 0021-9533 (print)
Related URLs:
Keywords: junction adhesion molecule, tight junction, trophectoderm epithelium, cell polarity, blastocyst, Adherens junction
Subjects: R Medicine > R Medicine (General)
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 26030
Date Deposited: 12 Apr 2006
Last Modified: 27 Mar 2014 18:14
URI: http://eprints.soton.ac.uk/id/eprint/26030

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