Novel osteoinductive biomimetic scaffolds stimulate human osteoprogenitor activity: implications for skeletal repair

Yang, X.B., Green, D.W., Roach, H.I., Clarke, N.M.P., Anderson, H.C., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O.C. (2003) Novel osteoinductive biomimetic scaffolds stimulate human osteoprogenitor activity: implications for skeletal repair. Connective Tissue Research, 44, (Supplement 1), 312-317. (doi:10.1080/713713599).


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The development of new bone formation strategies offers tremendous therapeutic implications in a variety of musculoskeletal diseases. One approach involves harnessing the regenerative capacity of osteoprogenitor bone cells in combination with biomimetic scaffolds generated from appropriate scaffold matrices and osteoinductive factors. The aims of our study were to test the efficacy of two innovative osteoinductive agents: the osteoblast stimulating factor-1 (osf-1), an extracellular matrix-associated protein, and osteoinductive extracts of Saos-2 cells on human osteoprogenitor cells. Saos-2 extracted osteoinductive factors significantly stimulated alkaline phosphatase specific activity in basal and osteogenic conditions. Osf-1 significantly stimulated chemotaxis, total colony formation, alkaline phosphatase-positive colony formation, and alkaline phosphatase specific activity at concentrations as low as 10 pg/ml compared with control cultures. Osteoinductive factors present in Saos-2 cell extracts and osf-1 promoted adhesion, migration, expansion, and differentiation of human osteoprogenitor cells on 3-D scaffolds. The successful generation of 3-D biomimetic structures incorporating osf-1 or osteoinductive factors from Saos-2 cells indicates their potential for de novo bone formation that exploits cell-matrix interactions.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1080/713713599
ISSNs: 0300-8207 (print)
Related URLs:
Keywords: biodegradable polymer, bone marrow, osteoprogenitor, plga, poly(-lactic-co- ÷ glycolic acid), tissue engineering
Subjects: R Medicine > RM Therapeutics. Pharmacology
Q Science > QP Physiology
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
ePrint ID: 26140
Accepted Date and Publication Date:
Date Deposited: 19 Apr 2006
Last Modified: 31 Mar 2016 11:48

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