The p36 isoform of BAG-1 is translated by internal ribosome entry following heat shock

Coldwell, Mark J., deSchoolmeester, Matthew L., Fraser, Graham A., Pickering, Becky M., Packham, Graham and Willis, Anne E. (2001) The p36 isoform of BAG-1 is translated by internal ribosome entry following heat shock. Oncogene, 20, (30), 4095-4100.


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BAG-1 (also known as RAP46/HAP46) was originally identified as a 46 kDa protein that bound to and enhanced the anti-apoptotic properties of Bcl-2. BAG-1 exists as three major isoforms (designated p50, p46 and p36 or BAG-1L, BAG-1M and BAG-1S respectively) and one minor isoform (p29), which are translated from a common transcript. The differing amino terminus determines both the intracellular location and the repertoire of binding partners of the isoforms which play different roles in a variety of cellular processes including signal transduction, heat shock, apoptosis and transcription. Although in vitro data suggest that the four BAG-1 isoforms are translated by leaky scanning, the patterns of isoform expression in vivo, especially in transformed cells, do not support this hypothesis. We have performed in vivo analysis of the BAG-1 5' untranslated region and shown that translation initiation of the most highly expressed isoform (p36/BAG-1S) can occur by both internal ribosome entry and cap-dependent scanning. Following heat shock, when there is a downregulation of cap-dependent translation, the expression of the p36 isoform of BAG-1 is maintained by internal ribosome entry.

Item Type: Article
Related URLs:
Keywords: bag-1, ires, translation, internal ribosome entry, heat shock
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Q Science > QH Natural history > QH426 Genetics
Divisions : University Structure - Pre August 2011 > School of Medicine > Cancer Sciences
ePrint ID: 26253
Accepted Date and Publication Date:
Date Deposited: 21 Apr 2006
Last Modified: 31 Mar 2016 11:49

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