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Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Segregation of genomes in polyploid tumour cells following mitotic catastrophe
Segregation of genomes in polyploid tumour cells following mitotic catastrophe
Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.
radial arrangement of genomes, link to microtubule-organising centre, de-polyploidisation, resuming of mitosis, mitotic catastrophe
1005-1011
Erenpreisa, J.
6bc35d80-54c7-432c-bf95-6e8bc2e70ecc
Kalejs, M.
da6c9cb7-b672-458b-b0fd-8e05d28cdd3c
Ianzini, F.
6ef29ab4-47a2-4932-899e-741a801ffbda
Kosmacek, E.A.
bfa7c3dd-933f-44f4-8722-055f35f3c764
Mackey, M.A.
e03a0509-48cd-4b40-b047-b36100626451
Emzinsh, D.
118ec097-5772-4bee-8eb6-ea5e8baae705
Cragg, M.S.
ec97f80e-f3c8-49b7-a960-20dff648b78c
Ivanov, A.
727a3fd7-8998-443c-8088-5696c12b270c
Illidge, T.M.
43b02dd8-7761-4781-b10b-877e26222508
Erenpreisa, J.
6bc35d80-54c7-432c-bf95-6e8bc2e70ecc
Kalejs, M.
da6c9cb7-b672-458b-b0fd-8e05d28cdd3c
Ianzini, F.
6ef29ab4-47a2-4932-899e-741a801ffbda
Kosmacek, E.A.
bfa7c3dd-933f-44f4-8722-055f35f3c764
Mackey, M.A.
e03a0509-48cd-4b40-b047-b36100626451
Emzinsh, D.
118ec097-5772-4bee-8eb6-ea5e8baae705
Cragg, M.S.
ec97f80e-f3c8-49b7-a960-20dff648b78c
Ivanov, A.
727a3fd7-8998-443c-8088-5696c12b270c
Illidge, T.M.
43b02dd8-7761-4781-b10b-877e26222508

Erenpreisa, J., Kalejs, M., Ianzini, F., Kosmacek, E.A., Mackey, M.A., Emzinsh, D., Cragg, M.S., Ivanov, A. and Illidge, T.M. (2005) Segregation of genomes in polyploid tumour cells following mitotic catastrophe. Cell Biology International, 29 (12), 1005-1011. (doi:10.1016/j.cellbi.2005.10.008).

Record type: Article

Abstract

Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.

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More information

Published date: 2005
Keywords: radial arrangement of genomes, link to microtubule-organising centre, de-polyploidisation, resuming of mitosis, mitotic catastrophe

Identifiers

Local EPrints ID: 26297
URI: http://eprints.soton.ac.uk/id/eprint/26297
PURE UUID: cae488cd-faec-41ef-a727-b95b9a487924
ORCID for M.S. Cragg: ORCID iD orcid.org/0000-0003-2077-089X

Catalogue record

Date deposited: 11 Apr 2006
Last modified: 16 Mar 2024 02:58

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Contributors

Author: J. Erenpreisa
Author: M. Kalejs
Author: F. Ianzini
Author: E.A. Kosmacek
Author: M.A. Mackey
Author: D. Emzinsh
Author: M.S. Cragg ORCID iD
Author: A. Ivanov
Author: T.M. Illidge

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