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Incidence of novel N-glycosylation sites in the B-cell receptor of lymphomas associated with immunodeficiency

Incidence of novel N-glycosylation sites in the B-cell receptor of lymphomas associated with immunodeficiency
Incidence of novel N-glycosylation sites in the B-cell receptor of lymphomas associated with immunodeficiency
Novel N-glycosylation sites are introduced by somatic mutation into the V genes of the majority of follicular lymphomas. Sites are positively selected and rare in normal memory B cells, indicating a potential role in tumour survival in the germinal centre (GC). The incidence of c. 40% in diffuse large B-cell lymphomas (DLBCL) parallels the known heterogenity of the disease. Immunodeficiency-related non-Hodgkin's lymphomas (NHL) include post-transplant lymphoproliferative disorders (PTLD) and acquired immunodeficiency syndrome-related NHL (AIDS-NHL). Most PTLD derive from B cells that carry mutated VH genes and that have completed the GC reaction. All AIDS-NHL carry mutated VH genes and variable features of GC or post-GC cells. To determine if N-glycosylation is a feature of immunodeficiency-related lymphomas, we analysed the VH genes of 19 PTLD and 36 AIDS-NHL. Novel sites were rare in PTLD (4/19), similar to memory B cells (P = 0·15). AIDS-NHL, including DLBCL and Burkitt's lymphomas (BL), showed heterogeneity with 16 of 36 (44%) having novel sites. The findings indicate no selection of N-glycosylation sites in PTLD, consistent with post-GC features. The variable incidence of N-glycosylation sites in AIDS-NHL mirrors that in DLBCL and sporadic BL of immunocompetent hosts, supporting the known heterogeneity of these disorders, and possibly pointing to distinct routes of tumour development.
0007-1048
604-609
Forconi, F.
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Capello, D.
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Berra, E.
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Rossi, D.
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Gloghini, A.
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Cerri, M.
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Muti, G.
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Morra, E.
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Paulli, M.
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Magrini, U.
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Lucioni, M.
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Rambaldi, A.
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Lauria, F.
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Carbone, A.
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Stevenson, F.K.
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Gaidano, G.
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Forconi, F.
ce9ed873-58cf-4876-bf3a-9ba1d163edc8
Capello, D.
c87f5af5-62d9-43dc-8b39-293f25873e53
Berra, E.
8cd59573-516d-42b4-bb56-f1b249c3b140
Rossi, D.
d690199a-0f3b-426b-9c3d-e50832cedf1c
Gloghini, A.
6b09a0af-897f-4715-b119-8d68ad2fdb46
Cerri, M.
fb913572-ad05-49d1-8210-cc1c1a57b0c4
Muti, G.
47797dda-1170-44d2-9b16-06f56da8002b
Morra, E.
813dff9c-6a63-4db0-900a-d61807a6e0dd
Paulli, M.
5f583b45-00f9-470a-9acf-d7a294df8948
Magrini, U.
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Lucioni, M.
c9e696bb-c8dc-49a2-bac7-34daaa919004
Rambaldi, A.
bfd1d3e3-8686-4afd-a958-f41a43aa8318
Lauria, F.
735fa868-573e-4a9b-b409-a3316d397c4a
Carbone, A.
f2c30ba1-94ce-4c7c-a5c8-5dbf67cd7d0a
Stevenson, F.K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Gaidano, G.
cbdc7c47-3fda-4b95-9161-65fb2afce974

Forconi, F., Capello, D., Berra, E., Rossi, D., Gloghini, A., Cerri, M., Muti, G., Morra, E., Paulli, M., Magrini, U., Lucioni, M., Rambaldi, A., Lauria, F., Carbone, A., Stevenson, F.K. and Gaidano, G. (2004) Incidence of novel N-glycosylation sites in the B-cell receptor of lymphomas associated with immunodeficiency. British Journal of Haematology, 124 (5), 604-609. (doi:10.1111/j.1365-2141.2004.04816.x).

Record type: Article

Abstract

Novel N-glycosylation sites are introduced by somatic mutation into the V genes of the majority of follicular lymphomas. Sites are positively selected and rare in normal memory B cells, indicating a potential role in tumour survival in the germinal centre (GC). The incidence of c. 40% in diffuse large B-cell lymphomas (DLBCL) parallels the known heterogenity of the disease. Immunodeficiency-related non-Hodgkin's lymphomas (NHL) include post-transplant lymphoproliferative disorders (PTLD) and acquired immunodeficiency syndrome-related NHL (AIDS-NHL). Most PTLD derive from B cells that carry mutated VH genes and that have completed the GC reaction. All AIDS-NHL carry mutated VH genes and variable features of GC or post-GC cells. To determine if N-glycosylation is a feature of immunodeficiency-related lymphomas, we analysed the VH genes of 19 PTLD and 36 AIDS-NHL. Novel sites were rare in PTLD (4/19), similar to memory B cells (P = 0·15). AIDS-NHL, including DLBCL and Burkitt's lymphomas (BL), showed heterogeneity with 16 of 36 (44%) having novel sites. The findings indicate no selection of N-glycosylation sites in PTLD, consistent with post-GC features. The variable incidence of N-glycosylation sites in AIDS-NHL mirrors that in DLBCL and sporadic BL of immunocompetent hosts, supporting the known heterogeneity of these disorders, and possibly pointing to distinct routes of tumour development.

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Published date: 2004

Identifiers

Local EPrints ID: 26313
URI: http://eprints.soton.ac.uk/id/eprint/26313
ISSN: 0007-1048
PURE UUID: c413d751-6aed-48a5-80af-65c0e62b7e72
ORCID for F. Forconi: ORCID iD orcid.org/0000-0002-2211-1831
ORCID for F.K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

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Date deposited: 24 Apr 2006
Last modified: 16 Mar 2024 04:09

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Contributors

Author: F. Forconi ORCID iD
Author: D. Capello
Author: E. Berra
Author: D. Rossi
Author: A. Gloghini
Author: M. Cerri
Author: G. Muti
Author: E. Morra
Author: M. Paulli
Author: U. Magrini
Author: M. Lucioni
Author: A. Rambaldi
Author: F. Lauria
Author: A. Carbone
Author: F.K. Stevenson ORCID iD
Author: G. Gaidano

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