The crystal structure Of H-2Db complexed with a partial peptide epitope suggests an MHC class I assembly-intermediate
Glithero, Ann, Tormo, Jose, Doering, Klaus, Kojima, Mayumi, Jones, E. Yvonne and Elliott, Tim (2006) The crystal structure Of H-2Db complexed with a partial peptide epitope suggests an MHC class I assembly-intermediate. The Journal of Biological Chemistry, 281, (18), 12699-12704. (doi:10.1074/jbc.M511683200).
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Description/Abstract
In the absence of bound peptide ligands, MHC class I molecules are unstable. In an attempt to determine the minimum requirement for peptide-dependent MHC class I stabilisation we have used short synthetic peptides derived from the Sendai Virus Nucleoprotein (NP) epitope (residues 324-332 1FAPGNYPAL9) to promote its folding in vitro of H-2Db. We found that H-2Db can be stabilised by the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of the optimal nonapeptide and includes both the P5 and P9 anchor residues. We have crystallised the complex of the H-2Db molecule with the pentamer and determined the structure to show how a quasi-stable MHC Class I molecule can be formed by occupancy of a single binding pocket in the peptide-binding groove.
| Item Type: | Article |
|---|---|
| ISSNs: | 0021-9258 (print) |
| Related URLs: | |
| Subjects: | R Medicine > R Medicine (General) Q Science > QR Microbiology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Cancer Sciences |
| Item ID: | 26325 |
| Date Deposited: | 07 Apr 2006 |
| Last Modified: | 02 Mar 2012 12:46 |
| Contributors: | Glithero, Ann (Author) Tormo, Jose (Author) Doering, Klaus (Author) Kojima, Mayumi (Author) Jones, E. Yvonne (Author) Elliott, Tim (Author) |
| Date: | 2006 |
| Status: | Published |
| Contact Email Address: | tje@soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/26325 |
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