Analysis of the oligomeric requirement for signaling by CD40 using soluble multimeric forms of its ligand, CD154


Haswell, Linsey E., Glennie, Martin J. and Al-Shamkhani, Aymen (2001) Analysis of the oligomeric requirement for signaling by CD40 using soluble multimeric forms of its ligand, CD154. European Journal of Immunology, 31, (10), 3094-3100. (doi:10.1002/1521-4141(2001010)31:10<3094::AID-IMMU3094>3.0.CO;2-F).

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Description/Abstract

We describe the construction of a novel soluble dodecameric form of CD154 (CD40 ligand) that is more effective than trimeric tCD154 in triggering B cell activation. Dodecameric surfactant protein (SP)-D-CD154 was more potent than tCD154 in inducing B cell proliferation over a wide range of concentrations. At saturating concentrations, the level of proliferation triggered by SP-D-CD154 was fourfold higher than that achieved with tCD154. Moreover, stimulation with dodecameric CD154 induced higher levels of the costimulatory molecules ICAM-1 and CD86. The higher activity of dodecameric CD154 when compared to trimeric CD154 is unlikely to be due to differences in their avidity for CD40, since both forms bound to CD40 strongly. Therefore, the extent of receptor clustering directlyregulates signaling by CD40.

Item Type: Article
Related URLs:
Keywords: TNF, TNF receptor superfamily, surfactant protein-D; B cell, receptor clustering
Subjects: R Medicine > R Medicine (General)
Q Science > QR Microbiology > QR180 Immunology
Divisions: University Structure - Pre August 2011 > School of Medicine > Cancer Sciences
ePrint ID: 26378
Date Deposited: 24 Apr 2006
Last Modified: 27 Mar 2014 18:15
Contact Email Address: aymen@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/26378

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