Interfacial properties of the M1 segment of the nicotinic acetylcholine recepter
Ambroggio, EE, Villarreal, MA, Montich, GG, Rijkers, DTS, de Planque, MRR, Separovic, F and Fidelio, GD (2006) Interfacial properties of the M1 segment of the nicotinic acetylcholine recepter. Biophysical Chemistry, 121, (3), 171-176.
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We have studied the thermodynamic, surface, and structural properties of αM1 transmembrane sequence of the nicotinic acetylcholine receptor (nAChR) by using Langmuir monolayer, FT-IR spectroscopy and molecular dynamics simulation techniques in membrane-mimicking environments. M1 spontaneously incorporates into a lipid-free air–water interface, showing a favourable adsorption free energy of − 7.2 kcal/mol. A cross-sectional molecular area of 210 Å2/molecule, a surface potential of 4.2 fV/molecule and a high stability of the film were deducted from pure M1 monolayers. FT-IR experiments and molecular dynamics simulations in membrane-mimicking environments (sodium-dodecyl-sulfate and CCl4, respectively) indicate coexistence between helical and non-helical structures. Furthermore, mixed peptide–lipid monolayers and monolayer penetration experiments were performed in order to study the peptide–lipid interaction. Mixed with condensed lipids (dipalmitoyl-phosphocholine, and dipalmitoyl-phosphoglycerol), M1 shows immiscible/miscible behaviour at low/high peptide concentration, respectively. Conversely, a complete miscible peptide–lipid interface is observed with liquid-expanded lipids (palmitoyl-oleoyl-phosphocholine, and palmitoyl-oleoyl-phosphoglycerol). Peptide penetration experiments demonstrate that the M1 peptide preferentially interacts with zwitterionic phosphocholine interfaces.
|Keywords:||Peptide monolayer; Peptide–lipid interaction; Peptide–lipid mixed monolayers; Nicotinic acetylcholine receptor; Gibbs adsorption free energy; Molecular dynamics simulation; Peptide secondary structure; Distorted helix; Fourier-transform infrared spectroscopy; Transmembrane αM1 peptide|
|Divisions:||Faculty of Physical and Applied Science > Electronics and Computer Science > NANO
|Date Deposited:||16 Oct 2007|
|Last Modified:||02 Mar 2012 13:21|
|Contributors:||Ambroggio, EE (Author)
Villarreal, MA (Author)
Montich, GG (Author)
Rijkers, DTS (Author)
de Planque, MRR (Author)
Separovic, F (Author)
Fidelio, GD (Author)
|Further Information:||Google Scholar|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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