Solid-State 17O NMR as a Probe for Structural Studies of Proteins in Biomembranes
Lemaître, V, de Planque, MRR, Howes, AP, Smith, ME, Dupree, R and Watts, A (2004) Solid-State 17O NMR as a Probe for Structural Studies of Proteins in Biomembranes. Journal of the American Chemical Society, 126, (47), 15320-15321.
Download
Full text not available from this repository.
Description/Abstract
We report the first example of 17O NMR spectra from a selectively labeled transmembrane peptide, 17O-[Ala12]-WALP23, as a lyophilized powder and incorporated in hydrated phospholipid vesicles. It is shown that at high magnetic field it is feasible to apply 17O NMR to the study of membrane-incorporated peptides. Furthermore, we were able to estimate distances within the selectively labeled WALP peptide, which represents a consensus transmembrane protein sequence. This work opens up new applications of 17O solid-state NMR on biological systems.
| Item Type: | Article |
|---|---|
| Related URLs: | |
| Divisions: | Faculty of Physical and Applied Science > Electronics and Computer Science > NANO |
| Item ID: | 264685 |
| Date Deposited: | 16 Oct 2007 |
| Last Modified: | 02 Mar 2012 14:05 |
| Contributors: | Lemaître, V (Author) de Planque, MRR (Author) Howes, AP (Author) Smith, ME (Author) Dupree, R (Author) Watts, A (Author) |
| Date: | December 2004 |
| Status: | Published |
| Further Information: | Google Scholar |
| URI: | http://eprints.soton.ac.uk/id/eprint/264685 |
Actions (login required)
 |
View Item |
