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Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia

Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia
Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia
Deletions from the derivative chromosome 9, der(9), of the translocation, t(9;22)(q34;q11), at the site of the ABL/BCR fusion gene, have been demonstrated by fluorescence in situ hybridisation (FISH), in both Philadelphia chromosome (Ph)-positive chronic myeloid leukaemia (CML) and acute lymphoblastic leukaemia (ALL). In CML they occur in 10?15% of cases and appear to indicate a worse prognosis, whereas in ALL, the situation is unclear. This study presents the findings of dual fusion FISH used to detect such deletions in a series of 27 BCR/ABL-positive childhood ALL patients. Metaphase FISH was essential for the accurate interpretation of interphase FISH signal patterns. Three cases (11%) had a single fusion signal, resulting from deletions of the der(9). Three other patients with variant translocations and one with an insertion, also had a single fusion, but with no evidence of deletions. Gain of a fusion in approximately one-third of patients indicated a second Ph, which appears to be a diagnostic marker of Ph-positive ALL. This study shows that the incidence of deletions from the der(9) in childhood ALL is at least as high as that reported for CML.
0887-6924
564-571
Robinson, H.M.
c406aa02-ca17-4ba1-9aa4-24bab6415fc0
Martineau, M.
812d24f4-7585-4467-892b-0acf3f429d3e
Harris, R.L.
cee44550-801a-48d2-b7de-03ee47c2ffb5
Barber, K.E.
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Jalali, G.R.
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Moorman, A.V.
af5027f3-4927-4e62-b1be-ac4bdcf6cd8f
Strefford, J.C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Broadfield, Z.J.
6fcde5f3-fc22-411a-938f-009b1869a68a
Cheung, K.L.
38af31e3-3be2-4a35-bcbb-7a6ac11ff9fd
Harrison, C.J.
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Robinson, H.M.
c406aa02-ca17-4ba1-9aa4-24bab6415fc0
Martineau, M.
812d24f4-7585-4467-892b-0acf3f429d3e
Harris, R.L.
cee44550-801a-48d2-b7de-03ee47c2ffb5
Barber, K.E.
bfb2e135-d223-44fe-a4b5-dcfa77000f05
Jalali, G.R.
2f888ad4-2751-49ef-ab7f-828a4b508563
Moorman, A.V.
af5027f3-4927-4e62-b1be-ac4bdcf6cd8f
Strefford, J.C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Broadfield, Z.J.
6fcde5f3-fc22-411a-938f-009b1869a68a
Cheung, K.L.
38af31e3-3be2-4a35-bcbb-7a6ac11ff9fd
Harrison, C.J.
9c9f6b47-8bfc-49dd-b156-74539b170291

Robinson, H.M., Martineau, M., Harris, R.L., Barber, K.E., Jalali, G.R., Moorman, A.V., Strefford, J.C., Broadfield, Z.J., Cheung, K.L. and Harrison, C.J. (2005) Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia. Leukemia, 19 (4), 564-571. (doi:10.1038/sj.leu.2403629).

Record type: Article

Abstract

Deletions from the derivative chromosome 9, der(9), of the translocation, t(9;22)(q34;q11), at the site of the ABL/BCR fusion gene, have been demonstrated by fluorescence in situ hybridisation (FISH), in both Philadelphia chromosome (Ph)-positive chronic myeloid leukaemia (CML) and acute lymphoblastic leukaemia (ALL). In CML they occur in 10?15% of cases and appear to indicate a worse prognosis, whereas in ALL, the situation is unclear. This study presents the findings of dual fusion FISH used to detect such deletions in a series of 27 BCR/ABL-positive childhood ALL patients. Metaphase FISH was essential for the accurate interpretation of interphase FISH signal patterns. Three cases (11%) had a single fusion signal, resulting from deletions of the der(9). Three other patients with variant translocations and one with an insertion, also had a single fusion, but with no evidence of deletions. Gain of a fusion in approximately one-third of patients indicated a second Ph, which appears to be a diagnostic marker of Ph-positive ALL. This study shows that the incidence of deletions from the der(9) in childhood ALL is at least as high as that reported for CML.

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Published date: 2005
Additional Information: Original manuscript, childhood all

Identifiers

Local EPrints ID: 26574
URI: http://eprints.soton.ac.uk/id/eprint/26574
ISSN: 0887-6924
PURE UUID: 03c08185-0ff6-4184-b412-dfcdf27899dc
ORCID for J.C. Strefford: ORCID iD orcid.org/0000-0002-0972-2881

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Date deposited: 12 Apr 2006
Last modified: 16 Mar 2024 03:40

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Contributors

Author: H.M. Robinson
Author: M. Martineau
Author: R.L. Harris
Author: K.E. Barber
Author: G.R. Jalali
Author: A.V. Moorman
Author: J.C. Strefford ORCID iD
Author: Z.J. Broadfield
Author: K.L. Cheung
Author: C.J. Harrison

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