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Identification and assembly of V genes as idiotype-specific DNA fusion vaccines in multiple myeloma

Identification and assembly of V genes as idiotype-specific DNA fusion vaccines in multiple myeloma
Identification and assembly of V genes as idiotype-specific DNA fusion vaccines in multiple myeloma
Tumor-specific markers are important in identifying and tracking malignant cells. In this regard, functionally rearranged immunoglobulin variable (V) region genes in B-cell tumors fulfill and extend these criteria. V genes provide signature motifs in tumor cells and can delineate critical features of the clonal history of the cell of origin. They also define a tumor-specific antigen, which can be targeted for immunotherapy. Our focus has been on using novel DNA fusion vaccines to induce antitumor immunity. Here, we describe in detail the methods for identifying tumor-derived V genes at the nucleotide level in the malignant plasma cells of multiple myeloma. We further present the methodology for assembly of tumor V genes as single-chain variable region fragments (scFv), fused in frame with an immunopotentiating nontoxic bacterial sequence, Fragment C (FrC) of tetanus toxin. These scFv.FrC DNA vaccines provide protection in myeloma models and are currently in clinical trials. The vaccines are patient specific and can be rapidly assembled for clinical use.
dna vaccine, multiple myeloma, idiotype, immunoglobulion variable region
1-59259-916-8
105-120
Humana Press
Sahota, Surinder S.
66c1457f-cba2-4c49-9c8c-fcee0748b6b8
Townsend, Mark
6a970ea8-291c-4d40-a53c-8def87e87fef
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Sahota, Surinder S.
66c1457f-cba2-4c49-9c8c-fcee0748b6b8
Townsend, Mark
6a970ea8-291c-4d40-a53c-8def87e87fef
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c

Sahota, Surinder S., Townsend, Mark and Stevenson, Freda K. (2005) Identification and assembly of V genes as idiotype-specific DNA fusion vaccines in multiple myeloma. In, Multiple Myeloma: methods and protocols. (Methods in Molecular Medicine, 113) Humana Press, pp. 105-120.

Record type: Book Section

Abstract

Tumor-specific markers are important in identifying and tracking malignant cells. In this regard, functionally rearranged immunoglobulin variable (V) region genes in B-cell tumors fulfill and extend these criteria. V genes provide signature motifs in tumor cells and can delineate critical features of the clonal history of the cell of origin. They also define a tumor-specific antigen, which can be targeted for immunotherapy. Our focus has been on using novel DNA fusion vaccines to induce antitumor immunity. Here, we describe in detail the methods for identifying tumor-derived V genes at the nucleotide level in the malignant plasma cells of multiple myeloma. We further present the methodology for assembly of tumor V genes as single-chain variable region fragments (scFv), fused in frame with an immunopotentiating nontoxic bacterial sequence, Fragment C (FrC) of tetanus toxin. These scFv.FrC DNA vaccines provide protection in myeloma models and are currently in clinical trials. The vaccines are patient specific and can be rapidly assembled for clinical use.

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More information

Published date: 2005
Keywords: dna vaccine, multiple myeloma, idiotype, immunoglobulion variable region

Identifiers

Local EPrints ID: 26588
URI: http://eprints.soton.ac.uk/id/eprint/26588
ISBN: 1-59259-916-8
PURE UUID: fd1b7801-5135-497c-b4ee-43d5c9cc45fc
ORCID for Freda K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

Catalogue record

Date deposited: 20 Apr 2006
Last modified: 23 Jul 2022 01:41

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Contributors

Author: Surinder S. Sahota
Author: Mark Townsend

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