An integrated multimodal acoustic particle manipulator and optical waveguide sensor

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Description/Abstract

An acoustic/optical/microfluidic system is presented for the manipulation of bead-tagged DNA and other biomolecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target bio-molecules can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multi-modal acoustic chamber is presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half and quarter-wave nodal planes the bead size can be determined, providing a means of multiplexing the detection.