Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs
Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs
The CD154/CD40 interaction is an important pathway of CD4 T cell help for CD8 T cell responses. In this study, we address the role of CD70, a member of the TNF superfamily and the ligand for the T cell costimulatory receptor CD27, in CD40-mediated priming of CD8 T cells. Using an agonistic anti-CD40 mAb to mimic the CD154/CD40 interaction we demonstrate that the priming of OT-I TCR transgenic or endogenous mouse OVA-specific CD8 T cells is critically dependent on CD70/CD27 interaction. CD70 blockade inhibited CD40-mediated clonal expansion of CD8 T cells and reduced the number of memory CD8 T cells generated. Furthermore, CD70 blockade during the initial priming of CD8 T cells inhibited the ability of memory CD8 T cells to expand in response to a second encounter with Ag. Our data indicate that CD70 expression on APCs plays a key role in CD40-dependent CD8 T cell responses.
6542-6546
Taraban, V.Y.
f73a6e99-d8a6-4f5e-b1d2-2952ce7627aa
Rowley, T.F.
9275c995-1a7e-4729-96f1-ea6338aae600
Al-Shamkhani, A.
0a40b3ce-9d71-4d41-9369-7212f0a84504
Cancer Research U.K. and Tenovus
1 December 2004
Taraban, V.Y.
f73a6e99-d8a6-4f5e-b1d2-2952ce7627aa
Rowley, T.F.
9275c995-1a7e-4729-96f1-ea6338aae600
Al-Shamkhani, A.
0a40b3ce-9d71-4d41-9369-7212f0a84504
Taraban, V.Y., Rowley, T.F. and Al-Shamkhani, A.
,
Cancer Research U.K. and Tenovus
(2004)
Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs.
Journal of Immunology, 173 (11), .
Abstract
The CD154/CD40 interaction is an important pathway of CD4 T cell help for CD8 T cell responses. In this study, we address the role of CD70, a member of the TNF superfamily and the ligand for the T cell costimulatory receptor CD27, in CD40-mediated priming of CD8 T cells. Using an agonistic anti-CD40 mAb to mimic the CD154/CD40 interaction we demonstrate that the priming of OT-I TCR transgenic or endogenous mouse OVA-specific CD8 T cells is critically dependent on CD70/CD27 interaction. CD70 blockade inhibited CD40-mediated clonal expansion of CD8 T cells and reduced the number of memory CD8 T cells generated. Furthermore, CD70 blockade during the initial priming of CD8 T cells inhibited the ability of memory CD8 T cells to expand in response to a second encounter with Ag. Our data indicate that CD70 expression on APCs plays a key role in CD40-dependent CD8 T cell responses.
Text
Journal_of_Immunology.pdf
- Version of Record
Restricted to Repository staff only
Request a copy
More information
Published date: 1 December 2004
Identifiers
Local EPrints ID: 26633
URI: http://eprints.soton.ac.uk/id/eprint/26633
ISSN: 0022-1767
PURE UUID: 8374bf66-29de-43ec-a16f-4daec4646dcc
Catalogue record
Date deposited: 20 Apr 2006
Last modified: 16 Mar 2024 03:02
Export record
Contributors
Author:
V.Y. Taraban
Author:
T.F. Rowley
Corporate Author: Cancer Research U.K. and Tenovus
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
