Monitoring kinetics in the artificial human asthmatic airway using electrical impedance spectroscopy

Sun, Tao, Molnar, Camelia, Davies, Donna and Morgan, Hywel (2008) Monitoring kinetics in the artificial human asthmatic airway using electrical impedance spectroscopy. 12th annual European conference on micro & nanoscale technologies for biosciences, Montreux, Switzerland. 16 - 19 Nov 2008.

Abstract

Asthma is an herited disorder, but the remarkable increases in prevalence worldwide over the last three generations can not be directly attributed to genetic susceptibility since there has been insufficient time for gene frequency changes to occur. Recognizing that asthma has an underlying genetic predisposition, human disease tissue-based in vitro models of asthma have been developed, focusing on the airway epithelium. However, these models are relatively simplistic, low throughout systems and are limited, not only by the availability of tissue, but also by the paucity of non-destructive and non-invasive tools to continuously monitor the behavior of the cells. Electrical impedance spectroscopy (EIS) is a non-invasive and label-free technique that can be used to probe and monitor the properties of cells, and has been recently developed for high speed single cell analysis. In this paper we describe an impedance-based cell sensing chip that is used to monitor the kinetics of tight junctions in the human bronchial epithelial cell monolayer.

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Contributors

Author: Hywel Morgan

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