Holographically encoded microparticles for bead-based assays
Birtwell, Sam, Banu, Shahanara, Zheludev, Nikolay and Morgan, Hywel (2009) Holographically encoded microparticles for bead-based assays. Journal of Physics D: Applied Physics, 42, (5), 055507.
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Description/Abstract
We demonstrate a re-writable, high capacity holographic encoding technique for multiplexed bead-based suspension assays. The microparticles are made from SU8 doped with a photochromic diarylethene dye and manufactured using multilayer photolithography and dry etching. Each particle is encoded with a unique hologram, whose diffraction pattern consists of bright and dark regions, representing a binary number that identifies the particle. Theoretically up to 1024 unique codes are available on a 100μm particle using this method, when the code is read with a standard 2/3" CMOS camera. Encoding capacities of 512 unique codes have been demonstrated on a 500μm SU8 particle. The code is thermally stable for 3 days at 25 ◦C, and once written, the code can be erased and re-written once whilst still remaining readable. The code can be written into the particle during an assay experiment (no pre-encoding is required) and requires simple optics for reading.
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Physical and Applied Science > Electronics and Computer Science > NANO |
| Item ID: | 267120 |
| Date Deposited: | 19 Feb 2009 11:05 |
| Last Modified: | 23 Aug 2012 03:30 |
| Contributors: | Birtwell, Sam (Author) Banu, Shahanara (Author) Zheludev, Nikolay (Author) Morgan, Hywel (Author) |
| Date: | 7 March 2009 |
| Status: | Published |
| Publisher: | Institute of Physics |
| Further Information: | Google Scholar |
| ISI Citation Count: | 1 |
| URI: | http://eprints.soton.ac.uk/id/eprint/267120 |
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