Single cell imaging using electrical impedance tomography
Sun, Tao, Tsuda, Soichiro, Zauner, Klaus-Peter and Morgan, Hywel (2009) Single cell imaging using electrical impedance tomography. At 4th IEEE international conference of Nano/Micro Engineered and Molecular Systems, Shenzhen, China, 05 - 08 Jan 2009.
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Description/Abstract
Electrical impedance spectroscopy is a non-invasive technology for characterizing the dielectric properties of biological tissues and cells. Electrical impedance tomography extends impedance measurements from one dimension to two or three dimensions. Impedance measurements are performed across multiple electrodes, mapping the conductivity distribution within an object. In this paper, electrical impedance tomography is used to image a multi-nucleated cell, Physarum polycephalum, growing on agar gel in a miniaturized chip containing a circular 16-electrode array. An impedance analyzer combined with a USB-controlled multiplexing circuit board is used to perform adjacent impedance measurements. An open source software, EIDORS is used for image reconstruction and the system is evaluated using finite element modeling. Experimentally, a preliminary reconstructed image of Physarum is shown. The system has the potential to monitor kinetics of cells culture.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Additional Information: | Event Dates: 5th- 8th, January |
| Divisions: | Faculty of Physical Sciences and Engineering > Electronics and Computer Science > NANO Faculty of Physical Sciences and Engineering > Electronics and Computer Science > Agents, Interactions & Complexity |
| Item ID: | 267590 |
| Date Deposited: | 18 Jun 2009 19:25 |
| Last Modified: | 23 Jul 2012 03:51 |
| Contributors: | Sun, Tao (Author) Tsuda, Soichiro (Author) Zauner, Klaus-Peter (Author) Morgan, Hywel (Author) |
| Date: | 6 January 2009 |
| Additional Information: | Event Dates: 5th- 8th, January |
| Status: | Published |
| Further Information: | Google Scholar |
| ISI Citation Count: | 0 |
| URI: | http://eprints.soton.ac.uk/id/eprint/267590 |
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