On-chip electrical impedance tomography for imaging biological cells
Sun, Tao, Tsuda, Soichiro, Zauner, Klaus-Peter and Morgan, Hywel (2010) On-chip electrical impedance tomography for imaging biological cells. Biosensors and Bioelectronics, 25, (5), 1109-1115. (doi:10.1016/j.bios.2009.09.036).
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Electrical impedance tomography is an imaging technology that spatially characterizes the electrical properties of an object. We present a miniaturized electrical impedance tomography system that can image the electrical conductivity distribution within a two-dimensional cell culture. A chip containing a circular 16-electrode array was fabricated using printed circuit board developing technology and used to inject current and to measure spatial voltage across the object. The signal stimulation and voltage data acquisition were performed using an impedance analyzer, operating in four-electrode mode. An open source software, EIDORS was used for image reconstruction. Finite element modelling was used to simulate the image reconstruction process by imaging two ellipsoidal phantoms in the circular 16-electrode array. The effect of the regularization parameter in the reconstruction algorithm and the influence from noise on the fidelity of the images has been numerically analyzed. Experimentally, we show reconstructed images of a multi-nuclear single cellular organism, Physarum Polycephalum, demonstrating the first step towards impedance imaging of single cells in culture. Our system provides a non-invasive lab-on-a-chip technology for spatially mapping the electrical properties of single cells, which would be significant and useful for diagnostic and clinical applications.
|Keywords:||electrical impedance tomography, cell culture, cell imaging, lab-on-a-chip|
|Subjects:||Q Science > QA Mathematics > QA75 Electronic computers. Computer science|
|Divisions:||Faculty of Physical and Applied Science > Electronics and Computer Science > Agents, Interactions & Complexity
Faculty of Physical and Applied Science > Electronics and Computer Science > NANO
|Date Deposited:||09 Oct 2009 09:58|
|Last Modified:||12 Sep 2012 11:28|
|Contributors:||Sun, Tao (Author)
Tsuda, Soichiro (Author)
Zauner, Klaus-Peter (Author)
Morgan, Hywel (Author)
|Date:||15 January 2010|
|Further Information:||Google Scholar|
|ISI Citation Count:||4|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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