Activation of human dendritic cells is modulated by components of the outer membranes of Neisseria meningitidis


Al-Bader, Tamara, Christodoulides, Myron, Heckels, John E., Holloway, Judith, Semper, Amanda E. and Friedmann, Peter S. (2003) Activation of human dendritic cells is modulated by components of the outer membranes of Neisseria meningitidis. Infection and Immunity, 71, (10), 5590-5597. (doi:10.1128/IAI.71.10.5590-5597.2003).

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Description/Abstract

Neisseria meningitidis serogroup B is a major cause of life-threatening meningitis and septicemia worldwide, and no effective vaccine is available. Initiation of innate and acquired immune responses to N. meningitidis is likely to be dependent on cellular responses of dendritic cells (DC) to antigens present in the outer membrane (OM) of the meningococcus. In this study, the responses of human monocyte-derived DC (mo-DC) to OM isolated from parent (lipopolysaccharide [LPS]-replete) meningococci and from a mutant deficient in LPS were investigated. Parent OM selectively up-regulated Toll-like receptor 4 (TLR4) mRNA expression and induced mo-DC maturation, as reflected by increased production of chemokines, proinflammatory cytokines, and CD83, CD80, CD86, CD40, and major histocompatibility complex (MHC) class II molecules.

In contrast, LPS-deficient OM selectively up-regulated TLR2 mRNA expression and induced moderate increases in both cytokine production and expression of CD86 and MHC class II molecules. Preexposure to OM, with or without LPS, augmented the allostimulatory properties of mo-DC, which induced proliferation of naive CD4+ CD45RA+ T cells. In addition, LPS-replete OM induced a greater gamma interferon/interleukin-13 ratio in naive T cells, whereas LPS-deficient OM induced the reverse profile. These data demonstrate that components of the OM, other than LPS, are also likely to be involved in determining the levels of DC activation and the nature of the T-helper immune response.

Item Type: Article
ISSNs: 0019-9567 (print)
Related URLs:
Subjects: R Medicine > RB Pathology
R Medicine > RF Otorhinolaryngology
Q Science > QR Microbiology
Divisions: University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
ePrint ID: 26902
Date Deposited: 25 Apr 2006
Last Modified: 27 Mar 2014 18:15
Contact Email Address: mc4@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/26902

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