Interleukin-4 and -13 expression is co-localized to mast cells within the airway smooth muscle in asthma
Brightling, C.E., Symon, F.A., Holgate, S.T., Wardlaw, A.J., Pavord, I.D. and Bradding, P. (2003) Interleukin-4 and -13 expression is co-localized to mast cells within the airway smooth muscle in asthma. Clinical and Experimental Allergy, 33, (12), 1711-1716. (doi:10.1111/j.1365-2222.2003.01827.x).
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Background: Airway smooth muscle infiltration by mast cells is a feature of asthma and not eosinophilic bronchitis. In asthma, Th2 cytokines have been implicated as playing a critical role in the development of airway inflammation and hyper-responsiveness. Whether inflammatory cells within the airway smooth muscle release these cytokines is unknown.
Methods: We have undertaken a comparative immunohistochemical study in bronchial biopsies from 14 subjects with asthma, 10 with eosinophilic bronchitis and eight normal controls recruited from two centres.
Results: The median number of IL-4+ cells/mm2 smooth muscle was significantly higher in subjects with asthma than eosinophilic bronchitis and normal controls for both the anti-IL-4 mAb 3H4 (2.4, 0, 0, respectively; P=0.001) and anti-IL-4 mAb 4D9 (1.6, 0, 0, respectively; P=0.02). There were no group differences in the number of IL-5+ cells (P=0.31). In six subjects with asthma, IL-13 expression by cells within the airway smooth muscle was studied. The median (range) of IL-13+cells was 2 (0.9–2.7). Ninety-four percent of the cells expressing IL-4 (3H4), 92% of those expressing IL-4 (4D9) and 100% expressing IL-13 in the airway smooth muscle were mast cells. Fifty-five percent of the mast cells within the airway smooth muscle co-localized to IL-4 (3H4), 29% to IL-4 (4D9) and 17% to IL-13.
Conclusions: In asthma, IL-4+ and IL-13+ cells were present within the airway smooth muscle and were expressed predominantly by mast cells, suggesting that IL-4 and IL-13 may play an important role in mast cell–airway smooth muscle interactions.
|Keywords:||airway hyper-responsiveness, airway smooth muscle, asthma, eosinophilic bronchitis, eosinophils, il-4, il-5, il-13, mast cells, th2, cytokines, t lymphocytes|
Q Science > QP Physiology
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
|Date Deposited:||25 Apr 2006|
|Last Modified:||01 Apr 2012 02:45|
|Contributors:||Brightling, C.E. (Author)
Symon, F.A. (Author)
Holgate, S.T. (Author)
Wardlaw, A.J. (Author)
Pavord, I.D. (Author)
Bradding, P. (Author)
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